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1、甘草甜素类药对化学损伤人肝细胞的保护作用比较重庆医科大学2009年第34卷第11期(JournalofChongqingMedicalUniversity2009.Vo1.34No.11)一1491一文章编号:0253—3626(2009)11-1491—04甘草甜素类药对化学损伤人肝细胞的保护作用比较张群,张丽峰2,陈鹏3,孙华丽s,张建军(1.东南大学附属中大医院感染病科,南京210009;2.成都中医药大学附属医院血管外科,成都610072;3.泸州医学院附属医院感染病科,泸州646000)【摘要】目的:研究4种(3类)甘草甜素药物对半
2、乳糖胺(D—GaIN)和四氯化碳(CC1)损伤培养人肝细胞(L一02)的保护作用及差异,筛选出疗效好的药物,指导临床应用.方法:培养L一02,用复方甘草酸单铵(Compoundammoniumglycyrrhizin,CAG),复方甘草酸苷(Compoundglyeyrrhizin,CG),甘草酸二铵(Diammoniumglycyrrihizinate,DG)和异甘草酸镁(Magnesiumisoglycyrrhizinate,MI)分别进行保护,再经D—GaIN或CC14处理.观察肝细胞生长状态,测定AST及LDH酶活力,测定细胞内谷胱甘
3、肽(GSH)含量.从而评价CAG,CG,DG和MI对D—GalN和cch损伤L一02细胞的保护作用.结果:浓度为1mg/ml时,CAG,CG,DG和MI均能显着抑制D—GaIN和CC1所致的AST及LDH释放,提高细胞的存活率.其中CAG抑制D—GaIN所致的AST效果显着优于CG,DG和MI(P<0.05);CAG抑制CC14所致的AST及LDH释放的效果显着好于DG和MI;CG抑制D—GaIN所致的AST和LDH释放效果显着(尸<0.05)好于MI.浓度为1mg/ml的CAG,CG,DG和MI4种药物均能显着抑制2种化学损伤细
4、胞内的GSH降低,其中CAG效果最明显(P<0.05).结论:1mg/ml的CAG,CG,DG和MI4种药物对D—GaiN和cch致人肝细胞损伤均有保护作用,其机制可能与抑制GSH降低相关.4种甘草甜素药物,CAG保护肝细胞效果最为显着,CG,DG和MI作用依次减低.【关键词】半乳糖胺;四氯化碳;甘草甜素;肝细胞;药物作用【中国图书分类法分类号】R965.1【文献标识码】A【收稿日期】2009—08—18ComparisonofprotectiveeffectofglycyrrhizindrugsOnhumanhepatocytesin
5、juredbyD-galactosamineandcarbontetrachloridezHANGOun,etal(DepartmentofInfectiousDiseases,feAffiliatedZhongdaHospital,SoutheastUniversity)【Abstract]Ojective:Toinvestigatetheeffectsandthedifferencesofthefour(3categories)glycyrrhizindrugsinprotectinghumanhepatocytes(L-02)fromt
6、heinjuryofgalactosamine(D—GaIN)andcarbontetrachloride(CCL),andtoscreenabetteroneforclinicalapplication.Methods:TheculturedL-02werepre-treatedwithcompoundammoniumglycyrrhizin(CAG),compoundglyeyrrhizin(CG),diammoniumglyeyrrihizinate(DG)andmagnesiumisoglycyrrhizinate(MI),respe
7、ctively,forprotectingthesecellsfromtheinjurymadewithCCI4orD-GaIN.Then,thelivercellgrowthstatuswereobserved,andtheASTandLDHenzymeactivityintheculturalsupernatantweredetermined,andcellularglutathione(GSH)contentintheculturalsupernatantweretested.Allthevaluesabovewereanalyzedt
8、ocomparetheprotectiveeffectsofthefourglycyrrhizindrugs.Results:Attheconcentrationo
