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1、细胞表面唾液酸及其连接方式对乳腺癌细胞黏附的影响作者：王晓玉1；林绍强2；李君武2；WolfgangKemmner3；丁彦青4 (暨南大学1第一医院妇产科，2医学院微生物与免疫学教研室，广东 广州 510630；3Max-DelbrueckCenterforMolecularMedicine，Berlin13125,Germany；4南方医科大学肿瘤学研究所，广东 广州 510515)摘要：目的 研究肿瘤细胞总唾液酸及α2，6-连接唾液酸对乳腺癌细胞MDA-MB435黏附力的影响。方法 乳腺癌细胞MDA-MB-435转染
2、顺义或反义α2，6唾液酸转移酶(ST6Gal-I)cDNA，以黑接骨木凝集素SNA筛选高、低表达α2，6唾液酸细胞克隆，检测各转染细胞的同源凝集作用，并比较唾液酸酶处理前后肿瘤细胞对胶原IV黏附力的变化。结果顺义转染克隆细胞-细胞之间的同源黏附能力下降，与胶原IV的黏附增强；反义转染克隆细胞-细胞之间同源黏附能力增强，而与胶原IV的黏附降低。唾液酸酶处理后肿瘤细胞与胶原IV的黏附力下降至未处理前的31%～57%。结论 乳腺癌MDA-MB435细胞表面唾液酸及α-2，6唾液酸化对肿瘤细胞的黏附有重要影响。关键词：乳腺肿瘤；唾液酸转移酶
3、；细胞外基质；细胞黏附；肿瘤转移中图分类号：R737 文献标识码：A 文章编号：1673-4254(2006)06-0742-05EffectofcellsurfacesialicacidandtheirlinkagesonadhesionofmammarycarcinomacellsWANGXiao-yu1；LINShao-qiang2；LIJun-wu2；KemmnerWolfgang3；DINGYan-qing4DepartmentofObstetricsandGynecology,FirstAffiliatedHospit
4、alofJi’nanUniversity1,DepartmentofMicrobiologyandImmunologyofMedicalCollege2,JinanUniversity,Guangzhou510630,China;3Max-DelbrueckCenterforMolecularMedicine，Berlin13125,Germany;4InstituteofOncology,SouthernMedicalUniversity,Guangzhou510515,ChinaAbstract:Objective Toinve
5、stigatetheeffectofcellsurfacesialicacidanditslinkageonthecell-cellandcell-matrixadhesionofmammarycarcinomacellsMD-MB-435.MethodsMD-MB-435cellsweresense-transfectedwithST6GalIcDNAorantisense-transfectedwithpartoftheST6GalIsequenceinsertedinpcDNA3.1vector,withmocktransfec
6、tionwithpcDNA3.1vectorasthecontrol.Thecellsurfaceα2,6-linkedsialylationwasdeterminedbyfluorescence-activatedcellsorting(FACS)usinglectinSNA(Sambucusnigraagglutininspecifictoα2,6-linkedsialicacidonN-linkedglycoprotein).Asignificantlyincreasedα2,6-sialylationsubcloneinsen
7、se-tranfectantsandadecreasedα2,6-sialylationsubcloneinantisense-transfectantswereselectedforfurtherexaminationofcell-cellandcell-matrix(collagenIV)adhesion.Thetransfectantswerealsotreatedwithsialidasetocomparethecapacityofcelladhesionaffectedbycellsurfacesialylation.Res
8、ultsSense-transfectionsubcloneshowedareducedcell-cellaggregationbutenhancedcell-matrixadhesion.Incontrast,thea
