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1、植物抗病基因工程研究进展论文.freeladesignificantprogressthatmolecularbiologyofplantdiseaseresistancemechanismsandgeicengineeringofplantdiseaseresistance.Theprinciple,targetinggenes,transformationmethodsandviemarized.Keyaionmethed;vie16。迄今人们已经从十余种不同植物中成功地克隆出了20多种R基因,如番茄抗叶霉病基因Cf2-97、Cf-28、Cf-49,水稻抗白叶枯病基因X
2、a2110,拟南芥的抗丁香假单胞杆菌基因RPS211、RPM112、抗霜霉病基因RPP513,亚麻抗锈病基因L614,大麦抗白粉病基因Mol15等。这些抗病基因多数已转化到相应的感病植株中,并均使转基因植株表现出了对病原菌特定生理小种的抗性。随着基因工程学研究的深入和发展,将获得大量的植物高密度遗传图谱,这为定位克隆技术的广泛应用提供了有利的条件和丰富的信息。同时由于R基因在序列和结构上的相似性,利用与已知R基因的同源关系分离新的R基因,将大大提高克隆效率与速度。2.2病原体无毒基因针对转基因植物抗性单一的问题,DeILTONRI.Defensestriggeredbypr
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