刺糖多孢菌基因工程菌构建与多杀菌素生物合成分析

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1、together,ametaboliclinkbetweenFAS,β-oxidationandspinosadbiosynthesisatthepresenceofexogenousfattyacidwasestablished.Theresultspresentedinthisstudyenableresearcherstobetterunderstandwhyfattyacidadditioncanincreasepolyketidesproduction.KEYWORDS：spinosad;Saccharop

2、olysporaspinosa;spinosadbiosynthesis;geneexpression;gene目录第一章文献综述........................................................................................................................11.1生物农药简介..................................................................

3、.................................................11.2多杀菌素概述...................................................................................................................21.2.1多杀菌素的发现及其结构......................................................................

4、.....21.2.2多杀菌素理化性质.................................................................................................31.2.3多杀菌素的生物活性.............................................................................................41.3多杀菌素生物合成途径及菌种改造..................

5、..............................................................61.3.1多杀菌素的生物合成途径.....................................................................................61.3.2S.spinosa合成多杀菌素所涉及的基因................................................................71.3.3

6、多杀菌素合成菌种改造.........................................................................................81.4刺糖多孢菌发酵过程研究................................................................................................91.5刺糖多孢菌生长微环境研究...................................

7、.......................................................101.6多杀菌素研究展望.........................................................................................................121.7本课题主要研究内容..........................................................................

8、...........................14第二章利用代谢工程逐步提高多杀菌素产量................................................................152.1前言......................................................