沙门菌pcr检测方法的建立与其初步应用

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1、扬卅I大学硕士学位论文4results．ItwasshownthatthePCRassaycoulddetectartificialSalmonellacontaminatedsamples，andthedetectionlimitwas43．85pgDNAor1CFUaftercultivation．TheseresultssuggestedthataspecificandsensitivePCRmethodWassuccessfullyestablished，andthisnewmethodprovidedausefulwayforthedetectionandmonit

2、oringofSalmonella．2．PreliminaryapplicationofthePCRassayforthedifferenttypesofsamplesFromJuly2008toJanuary2009，1082samplesofanusswab，liverandeggsfromavian，466samplesofanUSmop，meatandlymphnodefromswine，58watersamplesfrompoolandenvironmentinYangzhouregionweredetectedbythePCRassay,andSalmonella

3、Wasisolatedfromthepositivesamples．Resultsshowedthat：therewere41positivesoutofaviansamples，15positivesofswinesamples，2positivesoutofwatersamples．Wetotallygained58isolatesofSalmonella．ThePCRmethodWasverifiedbyabundancesamples，whichindicatedthatitWassuitablefordetectionofsamplesfromavian，swine

4、，water,etc，andapplicablefordifferenttypesofsampletestssuchastheanUSmop，liver,watersamples，meat．3．AntibioticresistanceofSalmonellaisolates100salmonellaisolatesweretestedfortheirsensitivityto18antimicrobialagentsbythediskdiffusionmethodrecommendedbyClinicalandLaboratoryStandardInstitutein2007

5、．TheresultsshowedthatthelevelofantibioticresistanceofSalmonellaishigh，andthesituationofmultipleantibioticresistanceofSalmonellaisnotoptimistic．Although100salomnellaisolateswerehi曲lysensitivetocephalosporins，theywerestronglyresistanttonalidixicacid，tetracyclineandstreptomycin．Studiesondrug-r

6、esistantspectrumshowedthatthemulti-drugresistanceof100isolatesrangingfrom2resistanceto13resistance，mainlyconcentratedinthe2resistanceto5resistanceand13resistance，whichaccountedfor78．13％．Thesedatashowedthatinourcountry,thephenomenonofmulti-drugresistanceofSalmonellaisveryserious．Theisolatesw

7、erehighlyresistanttoabroadspectrumofdrugsinourstudy,especiallyfourSalmonellaisolatesresistanceto13kindsofantibiotics．Inthisstudy,isolatesfromdifferentsourcesshoweddifferentsensitivityleveltodifferenttypesofantimicrobial．Therefore，itisimportanttochooserat