体外测定生物材料诱导成骨活性方法的建立和应用

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1、ESTABLISHMENTANDAPPLICATl0NOFANEWASSAYINVITROOFOSTEOINDUCTIVITYOFBIOMATER工ALSPostgraduateJIANGChuan-jieTutorQIJian-hongProfessorLUShi—biAcademicianSpecialitySurgeryABSTRACTLargebonedefectsandspinefusions，bonetumors，reconstructivesurgeryneedmassivebonegraftmaterials，butautogene

2、iebonegraftcan’tprovideelloughbonegraftsManyosteoconductiveandosteoinductivebonegraftsubstitutivematerialshadbeenusedinorthopedics．Thebioactivityofsomeosteoinductivebonegraftmaterialssuchasdemineralizedbonemarix，compositedmaterialscontainingnativebonemorphogeneticprotein，andot

3、herbioactivematerialscontainingrecombinedgrowthfactorsdependsontheactivityofgrowthfactorsinit，butthebioactivityofthesematerialsiseasytObeinactivatedbysometreatments，suchaspreparation,storage，sterilizationandSOon．Itisnecessarytoevaluateosteoinductivityofthesebioactivitematerial

4、sfortheeffectiveuseofbioactivematerials．Thetraditionalassaymethodinanimalbodyforevaluatingbioaetivityofmaterialshavemanyshortcomings，suchassacrificinganimals，longtermofassay,manyinterferencefactorstotestresult，standardizingandsequencingdifficultly，highcost，andSOon．ItCan’tsatis

5、fytheactualneed．Thisresearchwillestablishanewstandardizedassaymethodinvitroforevaluatingosteoinductivityofbioactivematerials．Thisassayshouldbeconvenient，repetitive，standardandlowcost，andCanreplacethetraditionalassaymethodinvivo．Thisresearchwillalsoattempttoassayosteoinductivit

6、yofkindsofbioactivematerialsandcompare、Ⅳimeachother．Objective：Toestablishanewstandardizedassaymethodinvitroforevaluatingosteoinductivityofbioactivematerials，whichisconvenient，repetitiveandlowcost，canreplacethetraditionalassaymethodinvivo．Besides，thisresearchwillattempttoassayo

7、steoinductivityofkindsofbioactivematerialsandcomparewitheachother．3Methods：Partone：EstablishmentofanewassayinvitroforevaluatingosteoinductivityofbioactivematerialsThegoalDBMwaspreparedusingfreshcorticalbonebyaserialofbiologicalandchemicaltreatments．BMP·2inthegoaldemineralizedb

8、onematrix(DBM)wasdeterminedbyimmunohistomicaltestGoalDBM(grou