丹参多酚酸盐对兔肢体缺血再灌注损伤保护作用的研究

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1、Theeffectofpolyphenolssaltonischemia·-reperfusioninjuryoftherabbitlimbsresearchAbstractObjective：ToobservetheinfluenceoftheSalvianolateonSerum‘flammatorvmediatc‘nterleukin8f也8)aadthromb(B2(anxB2)inflammatorymediatorsInterleuKlnarieltnromDoxaneADz),IlL舀JthecontentofTNFalpha，MalondialdehydeMDA)andsu

2、peroxidedismutase(SOD)duringtheLimbischemiareperfusionmodel．ObservetheinfluenceofSalvianolateonⅥ愀anddryweightratioofthemodel’Smuscle．PathologicalanalysisthemodeldamagedegreeoftheinterventionofSalvianolatetomusclefibers．Explorethemolecular．ResearchtheinterventioneffectanditsmechanismofactionofSalvi

3、anolateonlimbischemiareperfusioninjury．Methods：30NewZealandrabbitsweredividedtothreegroups．Onegroupisthenonoperafiongroup：onlythearteriesofrightlegsdon’tdotheischemicinjury．6hoursaftertheopermion，measurethelengthsofthemiddleoftherighthindlegs．After6hours，measurethemagainandcollectspecimen．Onegroup

4、isthemodelgroup：Pickoutthearteriesoftherighthindlegsduringtheoperations．Usingmicrovascularclipblockingfemoralartery,ligaturethepointofocclusionbyrubberrings．Blockingcollateralcirculationcausedbytherighthindlimbischemiamodelofrabbit．6hoursaftertheoperation，measurethelengthsofthemiddleoftherighthind

5、legs．Resumethebloodflowcausedbyischemiareperfusionmodel．Andreperfusionandafter6hours，measurethemagainandcollectSspecimen．Theothergroupistreatmentgroup：Methodofmakingmodellikethemodelgroup．6hoursaftertheoperation，measurethelengthsofthemiddleoftherighthindlegsandResumethebloodflow．BeforeRestorationo

6、fbloodflow(about1hour)，givethemanintravenousinjectionofSalvianolate(50mg／kg)．Payattentiontothem6hoursandmeasurethelengthsofthemiddleoftherighthindlegsandcollectspecimen．Calculatethepercentagereductionofthelengthsofthemiddleoftherighthindlegsofeachgroup．【(6h—alter一12h-alter)／6h-alterx1oo嘲；Cutthemid

7、dleofeachrighthindlegs’masscultstribalismanteriorandmusculargastronomiestodothewetanddryweightratiocheck．PullCtu-remeblood3-5mlininferiorvenacava．Bloodcoagulationby2000r／min．Thetwostepswithdoubleantibodysandwiche