多肽elisa方法建立及其在肝癌早期诊断相关研究中的初步应用

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1、安徽医科大学硕士学位论文目录缩略词表................................................................1摘要......................................................................2Abstract..................................................................4前言.........................

2、.............................................6第一章多肽Pep5单克隆抗体杂交瘤细胞株的建立.................131材料与仪器..............................................................131.1实验动物..............................................................131.2细胞系.......................

3、..........................................131.3免疫抗原.......................................................131.4主要试剂.......................................................141.5主要仪器.......................................................142实验方法.................

4、...............................................142.1动物免疫..............................................................142.2细胞融合..............................................................152.2.1制备小鼠骨髓瘤细胞悬液............................................152.2.2

5、制备饲养细胞层.....................................................152.2.3制备脾细胞..........................................................152.2.4细胞融合...........................................................152.3杂交瘤细胞上清抗体检测..........................................

6、....162.4阳性杂交瘤细胞的克隆.................................................162.5杂交瘤细胞的冷冻保存与复苏..........................................172.5.1杂交瘤细胞的冻存...................................................172.5.2杂交瘤细胞的复苏.................................................

7、..173结果与讨论..............................................................183.1小鼠尾血检测..........................................................18安徽医科大学硕士学位论文3.2细胞融合率............................................................183.3阳性杂交瘤细胞的初筛和复筛.................

8、........................193.4杂交瘤细胞株亚型鉴定................................................203.5上清单克隆细胞的传代................................................203.6上清单克隆抗体细胞株的冻存..........................