肢体缺血后处理对糖尿病大鼠局灶性脑缺血再灌注损伤的影响

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1、山东大学硕士学位论文awayfromadjacentnerves．ThedistalECAwasligatedandathreadwithroundedtipwasinsertedintoleftICAfromtheCCAandthreadedcraniallyuntilresistanceWasfelt(about18mm)whichensuredtheocclusionofthemiddlecerebralartery．ThethreadwasgentallywithdrawnfromtheICAtoallow

2、MCAreperfusion．SixtymaleSDratswereassignedto4groups：1)Controlgroup；2)Shamgroup：animalsweregiventhesameprocedurewiththeMCAOanimalswithoutinsertingthethread；3)I／Rgroup：thebrainswere：undergoneischemiafor90minfollowedbyreperfusionfor6hours；4)RPostCgroup．(2)Legisch

3、emicpostconditioning(RPostC)protocal：TheprocedureWasidenticaltoI／Rgroupduringwhichanimalsweresubjectedto3cyclesof5min／5minlegsischemia／reperfusion，andthelegswerebindedtightenouphtoensurethefemoralarterypulsationcannotbetouched．(3)Sixhoursafterreperfusion，allan

4、imalsweresubjectedtoneurologicalexaminationblindlyperformed．PerformanceWasscoredusingacommonlyusedscale(NDSldescribedbyLongaeta1．TheanimalswerethenkilledandTTCstainingWasusedtoobservethemorphousofinfarctionzoneanddeterminethechangesofbraininfarctvolume．Microsc

5、opeWasappliedtodetectthepathologicalmorphousofcerebralischemictissues．ThechangesofneuronalcellsapoptosiswereobservedthroughTUNELstainingmethodandtheultrastructureofmitochondriawereobservedbyelectronmicroscope．Ratbrainmitochondriawereisolatedandthelevelofmalond

6、ialdehyde(MDA)，Superoxidedismutase(SOD)，Na*，K+．ATPase，Ca2+．ATPase，andglutathioneperoxidase(GSH—Px)inmitochondriaWasmeasuredaccordingtothecommercialkitmanual．(4)Thedatawereexpressedasmean士standerrorwhiletheNDSWasexpressedasmedian．DataanalysisWasperformedusingth

7、eSPSS11．0．statisticalpackageprogramme。P

8、rolgroupandshamgroup，theNDSwassignificantlyincreasedinI／RgroupandRPostCgroup．ThereWasnosignificantdifferenceinNDSbetweenI／RandRPostCgroup(胗0．05)．(2)EffectsofRPostConcerebralinfarct