复合同种异体骨修复长段骨缺损的实验分析

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1、复合同种异体骨修复长段骨缺损的实验研究AbstractPurposesToinvestigatetheeffectoffreeautologousperiostealandvascularendothelialgrowthfactorcompoundwithallogeneicboneonthetreatmentofrabbitradiusdefects．MaterialsandMethodsFiveNewZealandwhiterabbits，weighing2．5．3kg，weresacrificedbyearvein

2、airembolism．Bilateralulnarbonewerehavestedwithsegmentlengthofabout40mm．andthentheywerecutinto3segments．eachl2mm．Exceptsofttissueandmarrow,aftersurface．demineralizedtheyweredeep．frozenin．800Cfortwoweeksandunfrozenforfutureuse．AnothersixtyNewZealandwhiterabbits，weig

3、hingis2．5．3kg．wereanesthetizedbysumianxininjection(0．15ml／kg)．afterremovingthehairanddisinfecingthebilateralalms．bonedefectof1．2cmwaswadeinthebilateralmiddleradialshaft．ThenthestandbyallogeneicboneWasplacedinthebonedefects．Finallyvascularendothelialgrowthfactorand

4、freeautologousperiostealwereapplicatedlocallyaloneorincombination．Therabbitsweredividedinto4Groups．Groupl(controlGroup)：allogeneicbone；Group2：boneailograftandfreeallograftperiosteumcompound；Group3：boneallograftandVEGFcompound；Group4：allograftbonewithVEGFandfreeaut

5、ologousperiostealcompound．Theresultswereassessedbygrossobservation．X．rayobservations，biomechanicalanalysisandhistologicalobservation(HEstaining1after4weeks、8weeks、l2weeks．Results1．grossobservation：after4weeks、8weeks、12weeks．bonecallusinbonedefectofGroup4iSmoreobvi

6、eousthanGroup1．2and3．Group3iSbetterthanGroup2and1．andGroup2iSbetterthanGroup1．After4and8weeks。callusofGroup4wassignificantlymorethan1．2and3，andthenewboneswerefoundinthebonedefectarea．2．X—rayexamination：Xraydisplayafter4and8weeks，BonycallusinGroup4WasmorethanGroup1

7、．2and3．Group3WasbetterthanGroupland2．ThereWasonlyasmallamountofnewboneformationinbonedefectinGroup1．Atier4weeks．somesofttissuefilledinit．Stillanonunionin8weeks．afterl2weeksboth4GroupsTobehealed．callusbonedefectsaresignificantlymorethanthehostbone，partofthemedullar

8、ycavityiSunobstructed．Theradiographicscoreafterstatisticalanalysisdisplaythatafter4weeks，themeanofradiographichealingscoreofGroup4ishigherthanGroup1,2，3