快速和延迟性远程缺血后处理对大鼠脑缺血保护作用分析

《快速和延迟性远程缺血后处理对大鼠脑缺血保护作用分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、andtheremoteischemicpostconditioningwasadministeredatthebeginningofreperfusionbywithdrawingmonofilamentsuture．(3)D-RIPGroup：themiddlecerebralarteryofratswasoccludedfor90min，andtheremoteischemicpostconditioningwasadministeredat6hoursafterreperfusionbywithdrawingmonofilamentsuture．Thebilateralfem

2、oralarteriesbluntlyseparated，atthebeginningofreperfusionor6hoursafterreperfusion，close／openthem10mininturnfor3circlesbyflogheartclip，namelyremoteischemicpostconditioning(RIP)．Functionalneurologicaloutcomeweremeasuredat1hourafterocclusion，12hours，24houm48hoursafterreperfusion，respectively．Atlast

3、ratsweresacrificedat48hoursafterreperfusion，theparaffin-embeddedtissueweretakentheinfarctsizeweremeasuredbyHEstain．Secondly,Inordertoinvestigatetheunderlyingneuroprotectivemechanismofrapiddelayedremoteischemicpostconditioning(R／D--RIP)onischemiaitsdifferences·Weselected24malehealthySDratsthenra

4、ndomlydividedinto4groups，6ratspergroup：(1)ShamGroup；(2)ControlGroup；(3)R-RIPGroup；(4)D—RIPGroup·Ratsweresacrificedat48hoursafterreperfusion，thenthespecimensweretaken．Wecompletetherelevantcellmolecularbiologyindexdetection．Results：(1)TheinfarctvolumemeasuredbyHEstainingshowthatcomparedwiththecon

5、tr01．R-RIPD-RIPgroupweresignificantlysmaller(P<0．05)；moreover,theinfarctvolumeofR-RIPgroupwasalsosignificantlysmallerthanR-RIPCgroupfP

6、IPandD—RIPgroupweresignificantlyhigherthanthecontrol(P<0．05)．Theneurologicalscoretestedat1afterocclusion，12hour,24hoursafterreperfusionofD—RIPgroupwaslowerthanR-RIPgroup，buttherewerenosignificantdifferentbetweenthetwogroups(P>0．05)；howevertheneurologicalscoretestedat48hoursafterreperfusionofD—R

7、IPgroupweresignificantlylowerthanR-RIPgroup(P<0．05)．Conclusions：Ourresultsimplythatbothrapidanddelayedremoteischemicpostconditioning(wo-RiP)coulddecreasetheinfarctvolumeofischemiainrats，andimprovetheneurologicalscore；moreoverR-RIP