dna的损伤和修复对多巴胺能神经元存亡的作用及其机制

《dna的损伤和修复对多巴胺能神经元存亡的作用及其机制》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、复旦大学博士后学位论文DNA的损伤和修复对多巴胺能神经元存亡的作用及其机制姓名：陈留记申请学位级别：博士后专业：神经生物学指导教师：Feng-yansun20040901ACKNoWLEDGEMENTSTheworkpresentedherewouldnothavebeenpossiblewithoutthesupport，adviceandhelpofmanypeople．Firstandforemost，Iamverygratefulthatmyadvisor，Dr，Feng—YanSun，gavem

2、etheopportunitytotakepartinthepostdoctoraltraininginherresearchprogram．1wouldliketothankherforprovidingmewithaproductiveandstimulatingenvironmentandforherconstantsupportthroughoutthisproject．Herenthusiasmandopen—mindednesshavemademypostdoctoralresearchane

3、xcitinganddelightfulexperience．IthankDr．Xue—JunLiforsharingherprimaryresearchresults．1wouldalsoliketoacknowledgescientificdiscussionwith，andoccasionalassistancefrom，mycollaboratorsonseveralofthestudiesdescribedherein：Prof，Yurt—QinGao，JingGu，Di·HanShen，Dr．

4、．Jian—HutLiu，Dr．Met—HongQiu，Shan—ZhenYangandYaoWang．ThisresearchhasbenefitedfromtheexcellenttechnicalsupportprovidedbyLing·MetZhang，Ya—linWang，Shi—DuoLuandYan-Liu．SimilarthanksarealsoduetoallmembersoftheNationalKeyLaboratoryofMedicalNeurobiology，pastandpr

5、esent，andindeed，toomanyothersattheShanghaiMedicalCollegeofFudanUniversity．Iameternallygratefulfortheunyieldingloveandsupportmyfamilieshaveshown箍ethroughoutmylife．1wouldespeciallytiketothankmywifeMing—FengJinforherconstantsupportthroughoutmystudies．Thisres

6、earchwassupportedbygrantsfromtheNationalKeyProjectofBasicScienceResearch(G1999054007)，NaturalScienceFoundation(30270479)andShanghaiMetropolitanFundforResearchandDevelopment(02JCl4025and04DZl4005)．6LISTofABBREVIATIONS8．OXOG8一hydroxyguanineBERBaseExcisionRe

7、pairDADopamineDAB3，3’一diaminobenzidine—tetrahydrochlorideDMEMDulbecco’SModifiedEagle’SMediumDMSODimethylSulphoxideECLEnhancedChemiluminescenceEDTAEthyleneDiamineTetraaceticAcidERCClExcisionRepairCrossComplementing1ETCElectronTransportChainFBSFetalBovineSe

8、rumFITCFluoresceinIsothiocyanateGSHReducedGlutathioneJC．15,5，6，6i-tetrachloro·1，1’，3，3’-tetraethylbenzimidaz010carbocyanineiodideKRHKerebs--Ringer·-HEPESMAOBMonoamineOxidaseBMPP+1-methyl一4一phenylpyridineionMPTP1-met