文昌鸡促卵泡素β亚基基因的单链核苷酸多态性及其和产蛋性能的相关性-研究

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1、文吕鸡促卵泡素B弧摹基因的单链核苷酸多态性及其’j产蛋性能的相关性研究(P<0．05)。18—39周的体重差异均不显著(P>O．05)。文昌鸡P1引物扩增获得2个等位基因，基因型AA和AB型的频率分别为O．49和0．51。两种等位基因型A和B的频率分别为0．74和0．26。经卡方适合性检验得知，文昌鸡内含子l处的基因型分布达到哈代．温伯格平衡状态(P>O．05)。关键词：文昌鸡；SNP；PCR-SSCP；rsnp基因；开产日龄22003)．Longlightstimulateschickengonadaldevelopment，andpromote

2、sexualmaturationinthechickenthroughtheopticnerve．LongnaturalsunshineinHainanleadstoearlierprecociouspubertyofWenchangchickens，andeggproductionrateofheniS5％at127d．Follicle·stimulatinghormone(Follicle—stimulatinghormone，FSH)issecretedbytheanteriorpituitaryglycoproteingonadotropi

3、ns，andplaysallimportantroleinthehypothalamus—pituitary—gonadalreproductiveaxis，whichiscomposedoftwosubunits，thecommona-subunitofhCG，LH，TSH，andotherglycoproteinhormonesandthespecific13-subunit，whichdeterminesthebindingtospecificreceptorsandinductionofbiologicalresponse，anditpla

4、ysakeyroleinhypothalamic—pituitary-gonadaxis．’’．Thepurposeofthisstudywastodetectsinglenucleotidepolymorphisms(SNPs)ofexons2and3and1，2intronwithinFSH[3gene，toanalyzethegeneticstructure，andtoinvestigatetheeffectsofthegeneonegglayingperformanceofWenchangchickens．Basedonthechichen

5、DNAsequencesofexons2and3and1，2intronwithinFSHl3geneinGenBank，threeprimerpairsforFSHf)gene，respectively,weredesignedtoscreenonenucleotidevariationofintron1bymeansofPCR-SSCPprotocolandanalyzetheassociationofpolymorphismswithearlyeggreproduction．Themainresultsareasshownasfollowin

6、g：3文昌鸡促卵泡素13亚基基因的单链核苷酸多态性及其与产蛋性能的相关性研究111eamplifiedfragmentwithinonepairsofthreeprimersshowedpolymorphism，andthepointmutationsofamplifiedsequenceinintronlofFSH[3gene．BasedonthesequenceofWenchangChickensFSHl3geneintronsl，specificamplificationsequenceweredesignedandPCR-SSCPanaly

7、siswasconductedunder。differentelectrophoreticconditions．neresultswerethattheprimersequenceWaspolymorphism，andgetthetwogenotypesAAandAB．SNPpolymorphisms(SNPs)．Wasgotbysequencing，andA_Cmutatio—noccurredin914ofintron1．mstatisticalanalysisofeggproductiontraitsandsNPpolymorphismofF

8、SHp。geneintrons1inWenchangChickensshowedthatSNPsinFSHl3genein