左金丸对应激性溃疡中枢调节的实验分析

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1、三、左金丸能够减少下丘脑室旁核内c，fos蛋白的表达，高剂量组与模型组有显著性差异。四、左金丸能够减少下丘脑室旁核内CRHmRNA的表达，左金丸高、中剂量组与模型组有显著性差异。五、左金丸能够降低血浆ACTH、CORT水平，抑制HPA轴活性，左金丸高剂量组接近正常对照组水平。综上实验结果表明：本研究在原有技术基础上改良的束缚浸水应激性溃疡模型，符合应激性溃疡的发病过程。模型组下丘脑PVN区c—los和CRHmRNA阳性细胞明显增多，且呈明显正相关关系；提示该区域在应激反应中具有关键作用，c—los参与调节CRHmRNA的表达及HPA轴的活性。左金丸能够抑制下丘脑PVN内c—fos

2、、CRHmRNA表达，降低血浆ACTH、CORT水平，升高胃液PH值，降低溃疡指数。左金丸防治应激性溃疡具有多重调节作用：能够直接干预应激系统，发挥中枢调节作用，从上游水平进行影响，抑制HPA轴的激活；进而抑制胃酸分泌，保护胃粘膜。雷尼替J一作为对照，对下丘脑PVN内c．fos蛋白及CRHmRNA的表达无调节作用，与模型组比较均无显著性差异；仅能抑制胃酸分泌，在胃粘膜局部发挥作用。左金丸对应激性溃疡多功能、多靶点干预，从应激系统上游水平发挥中枢调节作用，体现了巾医药整体调节的优势和特色。关键词；应激性溃疡左金丸c—fos中枢调节VExperimentalresearchonner

3、vecentralregulationbyZuojinpillonstressulcerName：ZhangHongmeiSupervisor：LiuXiaoweiABSTRACTBackground：Stressulcer(SU)isoneofthefamiliarstressdiseasesinclinic．ItisakindofacutediseasespecializedwithgastrointestinalmUCOUSandshallowulcer,whichwillcausebleedingandperforationofgastrointestinalmucous

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7、easuretheexpressionofproteinproductofc-fasandCRHmRNAinhippothalamicparaventricularnucleus，andsemmACTHandcortis01．Methods：MaleWistarratsaredividedinto6groups：normalcontrolgroup(A)；stressulcermodelgroup(B)；Zuojinpillwithhi曲dose(C1)；Zuojinpillwi