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1、HansJournalofAgriculturalSciences农业科学,2012,2,5-12doi:10.4236/hjas.2012.21002PublishedOnlineMarch2012(http://www.hanspub.org/journal/hjas)DetectionofEndosymbiontsinDifferentBemsiatabaci*PopulationsandTheirEffectsonWhiteflyHost#XiaXue,ShaojianLi,JujianChen,ShunxiangRen,BaoliQiuDepartmen
2、tofEntomology,SouthChinaAgriculturalUniversity,Guangzhou#Email:baileyqiu@scau.edu.cnReceived:Dec.7th,2011;revised:Dec.12th,2011;accepted:Dec.20th,2011Abstract:ThesweetpotatowhiteflyBemisiatabaci(Gennadius)isagloballydistributedagriculturalpest.Thespeciesandinfectionratesofdifferentend
3、osymbiontsinfieldandlaboratorypopulationsofB.tabaciBandQbiotyesweredetectedbymolecularmethods.Meawhile,theendosymbiontsinB.tabaciQbiotypelabpopulationwereinactivatedwithrifampicin,andthenthebiologyofWolbachiapositiveandnegativepopula-tionsofB.tabaciQbiotypewerecompared.Rusultsindicate
4、dthatallthepopulationsofB.tabaciwerein-fectedbytheprimaryendosymbiontCandidatusPortieraaleyrodidarum,regardlessbiotypeorpopulationhabitatoffieldorlab,whiletheinfectionofsecondaryendosymbiontsWolbachiaandArsenophonusweremarkeddifferentinvariousB.tabacipopulationsrelatedtobiotypeandhabi
5、tat.Inactivatedwith1.0mg/mlrifampicinfor48h,theinfectionofWolbachiainB.tabaciQbiotypewascompletelyeliminated,butnoob-viouseffectontheCandidatusandArsenophonuinallthewhiteflypopualtions.Treatedbyrifampicin,thefecundityoffemaleadultsreducedandthedevelopmentaltimeofF1offspringextended.Re
6、sultsalsoshowedthattheeliminationofWolbachiainB.tabaciledtotheincreaseofmaleprogenyproportion.Ingeneral,ourcurrentstudyshowedthatendosymbiontsvariedindifferentB.tabacipopulationsfromvarioushabitatsandWolbachiahassignificanteffectsonthebiologyofitswhiteflyhosts.Keywords:Endosymbiont;Ha
7、bitat;Bemisiatabaci;Antibiotic;BiologicalChange不同生境烟粉虱体内共生菌的检测及其*对寄主生物学特性的影响#薛夏，李绍建，陈驹坚，任顺祥，邱宝利华南农业大学资源环境学院昆虫学系，广州#Email:baileyqiu@scau.edu.cn收稿日期：2011年12月7日；修回日期：2011年12月12日；录用日期：2011年12月20日摘要：烟粉虱Bemisiatabaci是一种世界性分布的农业害虫。本文利用PCR扩增和DNA测序技术，研究了野生和实验室饲养的烟粉虱B型和Q型种群内共生菌的感染情况；并利用利福平对Q型烟粉
8、虱实验室种
