大鼠躯体感觉皮层锥体神经元至多极中间神经元突触上的长时程突触可塑性

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1、中国科学院神经科学研究所博士后士学位论文大鼠躯体感觉皮层锥体神经元至多极中间神经元突触上的长时程突触可塑性姓名：赵建平申请学位级别：博士后士专业：神经生物学指导教师：@2001.7.1桶墨咭c{}蛰1峙吁j、≮≈罨乏f铅力i／}匀、≮8圣{q≮岛争笔五皲叫“勾·扳咐铭豸痧(，厶刁，)弘妖岬鞘巧『，俘／r￡矽／圣叫l锹彳呻；《乏％乃必乏；2广／主@≠尹了云亏铭，夸父础芑妖坛争反(’谚友厍，∥芦吁名尹乞至多杨ff矽身叶纹己雾为世·Lb岳吲享f4筹匆钐可章r{(①f弘z矛宠．云f增I刁：塑垡亥务-s一

2、-／_,l,--一f'ff～扬刚謦一塑垆经乞磊彳历南、tf拿拿毛t繇L蔟厍ABS’I’RAC’l。Theactivityofinhibitoryinterneuronshasprofoundimpactontheoutcomeofneuralnetworks．Long-termpotentiation(LTP)andlong—termdepression(LTD)havebeenstudiedextensivelyatsynapsesbetweenexcitatoryneuronsHowever

3、，itisstillnotClearwhetherlong—termplasticityexistsinexcitatorysynapsesontoinhibitoryinterneurons．Inthepresentstudy，doublewhole—cellpatchclamprecordingsweremadeonpresynapticpyramidalneuronandpostsy,napticmultipolarGABAergicneuronsinL2／3somatosensoryco

4、rtexofP13-15ratbrainslices．Afterinductionb3correlatedpre-andpostsynapticspikes，theevokedunitaryEPSPamplitudeswerepersistentlyincreasedordecreaseddependingonthetimewindowbetweenpre—andpostsynapticspikes．Insomesynapses，theexpressionoflongtermplasticity

5、exhibitintheformofthefailureratechangesinevokedEPSP．Postsynapticapplication0fafastCa“bufferBAPTAaboIishedbothLTPandLTD．Bathperfusion0feitherNMDAreceptorantagonistAP5orL—typecalciumchannelblockernifedipinereversedLTPtoLTD．whilethesametreatmentshadnoef

6、fectonLTDinduction．ThepresentstudydemOnstrateSforthefirsttimetheexistence0fbothLTPandLTDinpyramidal-multipoplarinhibitoryneuronsynapsesinratsomatosensorycortex．Ourresultssuggestthatnovelmechanismsmaybeinvolvedintheinductionofthelong—termplasticityint

7、hispeculiartypeofsynapses．Keywords：long—termpotentiation；long—termdepressionpyramidalneuron：multipolarcortex；rat：doublewh01e—cellneuron：BAPTA：NMDAreceptorpatchclamprecording2Ithasbeenshownthatcorrelatedpre—andpostsynapticactivitycanproducelong—termch

8、angesinexcitatorytoexcitatoryneuronsynapsesincorticallayer2／3，layer4(Eggeretal，1999)，layer5(Markrametal，1997)．hippocampalCA3pyramidalneurons(Debanneetal，1998}andCMlturedhippocampalneurons(Bi&POO，1998)andthatbothpotentiationanddepressionofEPSPamplitud