堆肥微生物群落结构DGGE分析

《堆肥微生物群落结构DGGE分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、ApplMicrobiolBiotechnolDOI10.1007/s00253-012-4124-yENVIRONMENTALBIOTECHNOLOGYImpactofPhanerochaetechrysosporiuminoculationonindigenousbacterialcommunitiesduringagriculturalwastecompostingJiachaoZhang&GuangmingZeng&YaoningChen&ManYu&HongliHuang&ChangzhengFan&YiZhu&HuiL

2、i&ZhifengLiu&MingChen&MinJiangReceived:22March2012/Revised:18April2012/Accepted:18April2012#Springer-Verlag2012AbstractThisresearchwasconductedtodistinguishbetweencoincidedwithpiletemperature,ammonium,andnitrate(P

3、0.006).VariancepartitionanalysisshowedthattheP.chryso-ulationandsamplepropertyheterogeneityinducedbydifferentsporiuminoculationdirectlyexplained20.5%(P00.048)ofinoculationregimesontheindigenousbacterialcommunitiesthevariationinthebacterialcommunities,whereasthesampled

4、uringagriculturalwastecomposting.P.chrysosporiumwaspropertychangesinducedbydifferentinoculationregimesinoculatedduringdifferentphases.Thebacterialcommunityindirectlyexplainedupto35.1%(P00.002).ThebacterialabundanceandstructureweredeterminedbyquantitativePCRcommunityst

5、ructurewassignificantlyrelatedtopiletempera-anddenaturinggradientgelelectrophoresisanalysis,respec-ture,water-solublecarbon(WSC),andC/NratiowhenP.tively.ResultsindicatedasignificantstimulatoryeffectofP.chrysosporiumwereinoculated.TheC/Nratiosolelyexplainedchrysosporiu

6、minoculationonthebacterialcommunityabun-7.9%(P00.03)ofthevariationincommunitystructure,where-dance.ThebacterialcommunityabundancesignificantlyaspiletemperatureandWSCexplained7.7%(P00.026)and7.5%(P00.034)ofthevariation,respectively.P.chrysospo-J.Zhang:G.Zeng(*):Y.Chen(

7、*):C.Fan:Y.Zhu:Z.Liu:riuminoculationaffectedtheindigenousbacterialcommunitiesM.Chen:M.Jiangmostprobablyindirectlythroughincreasingpiletemperature,CollegeofEnvironmentalScienceandEngineering,enhancingthesubstrateutilizability,andchangingotherHunanUniversity,Changsha410

8、082,Chinaphysico-chemicalfactors.e-mail:zgming@hnu.edu.cne-mail:cyn@hnu.edu.cnKeywordsComposting.Phanerochaetechrysosporium.