资源描述:
《模拟酸雨对菲律宾樟树幼苗抗氧化系统的影响》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、En-ULiu&Chiung-PinLiu.EffectsofSimulatedAcidRainontheAntioxidativeSysteminCinnamomumphilippinenseSeedlings[J].WaterAirSoilPollut,2011,215(1):127–13.EffectsofSimulatedAcidRainontheAntioxidativeSysteminCinnamomumphilippinenseSeedlings模拟酸雨对菲律宾樟树幼苗抗氧化系统的影响AbstractThisstudyexaminedtheimpor
2、tanceoftheantioxidativedefensemechanismduringtheapplica-tionofsimulatedacidrain(SAR)pH4,pH3,pH2,andpH6ascontroltreatmentinCinnamomumphilippinenseseedlings.Analysiswascarriedouton1,10,and15daysofsprayingSAR.Inourresults,catalase(CAT),ascorbateperoxidase(APx),guaiacolperoxidase(GPOD),an
3、dglutathionereductase(GR)activitysignificantlywereinducedon1dayofsprayingSARpH3andpH2,suggestingthatC.philippinenseseedlingsexposedtopH3andpH2acidrainforonly1daywereunderoxidativestress,andantioxidantenzymewereapparentlyincreaseduntil10daysofsprayingSARpH4.However,on10daysofsprayingSA
4、RpH2,theaccumulatedintensityofaciditysignificantlyinhibitedtheactivi-tiesofCATandAPxaccompanyingwithincreasedconcentrationsofmalonydialdehyde(MDA).Onthecontrary,GPODactivityandtheascorbicacidratiowereremarkablyincreasedwhensprayingSARpH2for15days.Therefore,GPODandascorbatecontributeto
5、thescavengingofROSatstrongeraciditystresses.However,theywerenotenoughtoavoidcellulardamage,sincemembranelipidswereinjured,andnecrosisappeared.KeywordsAntioxidantenzyme.Cinnamomumphilippinense.Lipidperoxidation.Simulatedacidrain图1:用pH=6(对照组)的蒸馏水,pH值=4,pH=3,pH=2模拟酸雨,对菲律宾樟树幼苗叶片喷涂1天、10天、1
6、5天后,其过氧化氢酶活性的变化。用符号(*),(**),(***)分别表示差异分别控制在P<0.05,P<0.01,P<0.001。图2:用pH=6(对照组)的蒸馏水,pH值=4,pH=3,pH=2的模拟酸雨,对菲律宾樟树幼苗叶片喷涂1天、10天、15天后,其抗坏血酸过氧化物酶活性的变化。统计意义如图1所示。图3:用pH=6(对照组)的蒸馏水,pH值=4,pH=3,pH=2的模拟酸雨,对菲律宾樟树幼苗叶片喷涂1天、10天、15天后,其谷胱甘肽还原酶活性的变化。统计意义如图1所示。图4:用pH=6(对照组)的蒸馏水,pH值=4,pH=3,pH=2的模拟酸雨,对菲律宾
7、樟树幼苗叶片喷涂1天、10天、15天后,其愈创木酚过氧化物酶活性的变化。统计意义如图1所示。图5:用pH=6(对照组)的蒸馏水,pH值=4,pH=3,pH=2的模拟酸雨,对菲律宾樟树幼苗叶片喷涂1天、10天、15天后,其脂质过氧化产物丙二醛活性的变化。统计意义如图1所示。表1:喷洒15天pH=6(对照)的蒸馏水,pH=4、pH=3、pH=2的模拟酸雨对菲律宾樟树幼苗叶片中ASA、DHA、总ASA浓度,抗坏血酸比的影响。数值是指平均值±偏差,样本数=6。相关差异控制在P<0.05(*);P<0.01(**);P<0.001(***)。aASA和DHA鲜重浓度单位1μ
8、molg−