地黄寡糖在2型糖尿病大鼠模型上的降血糖作用与机制分析

《地黄寡糖在2型糖尿病大鼠模型上的降血糖作用与机制分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、兰州火学硕士学位论文HypoglycemiceffectofRehmanniaglutinosa01吨osaccharidesintype2diabeticratsanditsmechanismAbstractobjeetive：Thisthesisfocusesonestablishmentandevaluationofthebestmethodforinducingratmodeloftype2diabetesmellitus，andinvestigationoftheeffectofRehmanniaglutinosaoligosaccharide(ROS)intyp

2、e2diabeticratsandbyperglycaemiainnormalratsanditsmechanismMethods：ExperimentaldiabeticmodelswereinducedbyALXatthedoseofi50mg·kg一1byintraperitonealinjection(i．P．)，bothhighlipidfoodandlowdose(30mg-kg’1)ofSTZbyintravenousinjection(i．、，)，thesingleintraperitonealinjectionofSTZ(newlybornrats，90m

3、g’k91)，orbothhighlipidfoodandlowdoseofSTZ(30mg·kg’1)byintraperitonealinjection，respectively．Andplasmaglucose，bodyweightanddeathrateweredeterminedtoselectthebestmethodforinducingtype2diabeticratsmodel．rype2diabeticratsmodelwassystematicallyevaluatedinbodyweight，plasmaglucose，hepaticglycogen

4、，plasmalipids，weightsofinternalorgans，aswellasinsulinanditscounter-regulatoryhormonelevels(glucagonsandcorticosterone)．Accordingtothepurposeoftheexperiment，type2diabeticmodelwasinducedbybothhighlipidfoodandlowdoseofSTZ(30mg‘kg。1)inrats，hyperglycaemiainnormalratswasinducedbyglucose(2．5g,kg～

5、，i．P，)oradrenaline(300腭’kg一，i．P)．Theanimalsweredividedintocontrolgroup，type2diabeticmodelgrouporhyperglycaemiamodelgroup，ROS(100，200mg’kg-1．d“)treatedgroupandmetformin(200mg‘kg-1．d。1)treatedgroup．Alldrugswereadiministeredbyintragastricadministration(i．g．)．Resuits：Itwasfoundthathyperglycaem

6、iacouldbeinducedbyALX(i．p．)withagreatlydecreasedbodyweight．Hyperglycaemiacouldalsobeinducedbybothhi曲lipidfoodandlowdoseofSTZ(i．V．)，butwithoutgoodregularity．HyperglycaemiainducedinnewlybornratsbySTZ(i．P．)wasnotfeasiblebecauseoflowachievementratio，longperiodandhighdeathrate．Onlythehyperglyca

7、emiainducedbybothhighlipidfoodandlowdoseofSTZ(i．P．)wasstabileandwithlessinfluenceonbodyweightandthedeathratewasnotSOhighTheevaluationsof兰卅l大学顶上学位论文thismethodwereasfollows：bodyweightdecreasedatfirsttime，butincreasedlater；plasmaglucosewasstabileandthenincreasing