猪源肠外致病性大肠杆菌亚单位疫苗的研制

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1、HUAZHONGAGRICULTURALUNIVERSITY硕士学位论文MASTER’SDEGREEDISSERTATION猪源肠外致病性大肠杆菌亚单位疫苗的研制THERESEARCHOFPORCINEEXTRAINTESTINALPATHOGENICE.COLISUBUNITVACCINE研究生:马利芳CANDIDATE:MALIFANG导师:徐卓菲副教授SUPERVISOR:ASSOCIATEPROFESSORXUZHUOFEI汤细彪博士DOCTORTANGXIBIAO学位类型:兽医硕士DEGR

2、EETYPE:MASTEROFVETERINARY领域:FIELD:中国武汉WUHAN,CHINA二O一四年六月JUNE,2014万方数据学校名称：华中农业大学单位代码：10504分类号：密级：华中农业大学硕士学位论文猪源肠外致病性大肠杆菌亚单位疫苗的研制TheResearchofPorcineExtraintestinalPathogenicE.coliSubunitVaccine研究生:马利芳学号：2012302120046指导教师:徐卓菲副教授谭臣副教授汤细彪博士学位类型:兽医硕士领域：华中农

3、业大学动物医学院中国·武汉CollegeofVeterinaryMedicineHuazhongAgriculturalUniversityWuhan,China万方数据万方数据猪源肠外致病性大肠杆菌亚单位疫苗的研制目录摘要................................................................................................................................iAbstract...

4、............................................................................................................................iii缩略语表（Abbreviation）................................................................................................v1前言.......

5、...........................................................................................................................11.1肠外致病性大肠杆菌概述....................................................................................11.2大肠杆菌疫苗研究现状................

6、........................................................................31.3OmpC和OmpF蛋白研究现状............................................................................41.4毕赤酵母表达概述.........................................................................

7、.......................62研究目的与意义..............................................................................................................83材料与方法.......................................................................................................

8、...............93.1试验材料................................................................................................................93.1.1菌株质粒与培养基......................................................................................9