下调CD59的表达可以抑制HeLa细胞增殖并促进其凋亡.pdf

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1、细胞与分子免疫学杂志(ChinJCellMolImmuno1)2014，30(6585·论著·文章编号：1007—8738(2014)06—0585—03下调CD59的表达可以抑制HeLa细胞增殖并促进其凋亡高美华，李营，张蓓，王冰，梁洁，李园园(青岛大学医学院免疫教研室，山东青岛266071)[摘要]目的采用RNA干扰与噬菌体肽库展示技术2种方法干预宫颈癌HeLa细胞CD59的表达，以探讨比较2种CD59低表达方式对HeLa细胞增殖与凋亡的影响。方法将10~g／mL的CD59短肽封条作用于He

2、La细胞8h后，联合CD59干扰质粒pSUPER-siCD59稳定转染HeLa细胞系，采用MTI"法检测各组细胞增殖率的变化，采用TUNEL染色、annexinV—PE／7一AAD染色结合流式细胞术检测细胞凋亡。结果CD59短肽封条组与pSUPER．siCD59质粒稳定转染细胞组HeLa细胞增殖明显弱于对照组，TUNEL及流式细胞术检测结果显示2组处理细胞均存在细胞凋亡现象，且短肽封条的效果要好于pSUPER—siCD59质粒。结论下调CD59表达能抑制其细胞增殖并促进其凋亡，且短肽封条效果要优

3、于CD59RNA干扰质粒。[关键词]CD59；RNA干扰；短肽封条；HeLa细胞；增殖；凋亡[中图分类号]R392．11，Q253，R737．33[文献标志码]ADown-regulationofCD59inhibitsproliferationandpromotesapoptosisofHeLacellsGAOMeihua，LIYing，ZHANGBei，WANGBing，LIANGJie，LIYuanyuanDepartmentofImmunology，MedicalCollege，Qing

4、daoUniversity，Qingdao266071，ChinalAbstractjObjeefiveTointervenetheexpressionofCD59oncervicalcancerHeLacellsbyRNAinterferenceandphagedisplayrandompeptidelibrary，respectively，anddetecttheefectofdown-regulatedCD59expressiononproliferationandapoptosisoft

5、hecervicalcancercels．MethodsHeLacellsweredividedintonormalcontrolgroup．CD59peptidesealgroup．pSUPERtransfectedgroupandpSUPER-siCD59transfectedgroup．TheCD59peptidesealgroupwastreatedwith10ug／mLCD59peptidesealfor8hours．ThepSUPERandpSUPER-siCD59transfect

6、edgroupsweretransfectedwithemptyplasmidpSUPERandrecombinantplasmidpSUPER-sjCD59．respectively．M1-IassaywasusedforthedetectionofcelJproliferation．TUNEL．annexinV．PE／7．AADstainingcombinedwithflowcytometrywereadoptedforthedetectionofcelIapoptosis．ResultsC

7、omparedwiththecontrolgroups，bothCD59peptidesealgroupandpSUPER-siCD59transfectedgrouppresentedadiminishedcellproliferationactivityandastrengthenedapoptosis．What’Smore，theefectofpeptidesealonCD59expressioninhibitionwasbe~erthanthatofpSUPER-siCD59plasmi

8、d．CondusionDown-regulationofCD59expressioncouldinhibitHeLacells’proliferationandpromoteapoptosis，andtheinhibitoryefectofpeptidesealwasbe~erthanthatofCD59interferenceplasmid．[Keywords]CD59；RNAinterference；peptideseal；HeLacel；proliferation；apoptosis在肿瘤