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1、2016,35(10):1881-1887农业环境科学学报2016年10月允燥怎则灶葬造燥枣粤早则燥-耘灶增蚤则燥灶皂藻灶贼杂糟蚤藻灶糟藻曹书苗,王文科,王非,等.放线菌Act12对Pb胁迫下黑麦草根系生长及抗性生理的影响[J].农业环境科学学报,2016,35(10):1881-1887.CAOShu-miao,WANGWen-ke,WANGFei,etal.EffectsofactinomycetesAct12ontherootgrowthandresistantphysiologyofperennialryegrassunderPbstress[J
2、].JournalofAgro-EnvironmentScience,2016,35(10):1881-1887.放线菌Act12对Pb胁迫下黑麦草根系生长及抗性生理的影响1121曹书苗,王文科,王非,张军(1.长安大学旱区水文与生态效应教育部重点实验室,西安710054;2.空军工程大学理学院,西安710054)摘要:采用菌剂接种及盆栽实验,研究了不同水平Pb胁迫条件下放线菌Act12对黑麦草根系生长和抗性生理的影响。结果显示,土壤Pb胁迫水平不同,对黑麦草根系生长和生理特性的影响不同。接入放线菌后,黑麦草根分蘖和根鲜重较对照增加了12.3%~44.2
3、%和19.2%~94.6%,对根系生长有显著的促进作用;接菌后根系过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性较对照也有所增加,尤其在高浓度Pb胁迫下(500~1000mg·kg-1)对3种酶的促进作用最显著;接菌显著降低了根系丙二醛(MDA)含量,显著增加了根系活力。研究表明,Pb胁迫下,放线菌Act12的接入可增强根系抗氧化酶活性,降低质膜过氧化损伤,提高根系活力和根系对Pb胁迫的耐受性,进而显著促进黑麦草根系生长。关键词:Pb胁迫;放线菌;黑麦草;根系生长;抗性生理中图分类号:X171.5文献标志码:A文章编号:1672
4、-2043(2016)10-1881-07doi:10.11654/jaes.2016-0302EffectsofactinomycetesAct12ontherootgrowthandresistantphysiologyofperennialryegrassunderPbstressCAOShu-miao1,WANGWen-ke1,WANGFei2,ZHANGJun1(1.KeyLaboratoryofSubsurfaceHydrologyandEcologicalEffectsinAridRegion(MinistryofEducation),Ch
5、ang忆anUniversity,Xi忆an710054,China;2.CollegeofScience,AirForceEngineeringUniversity,Xi忆an710054,China)Abstract:AninoculationpotexperimentwasconductedtoinvestigatetheeffectsofactinomycetesAct12onthegrowthandresistantphysi-ologyofperennialryegrassunderPbstress.Resultsshowedthatdiff
6、erentlevelsofPbstressaffectedthegrowthandphysiologicalcharacter-isticsofryegrassrootstovariousdegrees.However,wheninoculatedwithactinomycetestheroottillernumberandrootfreshweightwerein-creasedby12.3%~44.2%and19.2%~94.6%,respectivelycomparedtocontrol,indicatingthatactinomycetescou
7、ldsignificantlypromoterootgrowth.Actinomycetes-inoculationincreasedrootenzymeactivitiesofcatalase(CAT),superoxidedismulase(SOD)andperoxidase(POD)comparedtocontrolaswellparticularlyinhighlevelPbstress(500~1000mg·kg-1).Moreover,actinomycetes-inoculationde-creasedrootmalondiadehyde(
8、MDA)contentandincreasedrootactivitysigni