盐霉素对人骨肉瘤MG-63细胞体外增殖和凋亡的影响.pdf

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1、昆明医科大学学报2014，35(7)：59～62CN53—1221／RJournalofKunmingMedicalUniversity盐霉素对人骨肉瘤MG一63细胞体外增殖和凋亡的影响张健”，李斌，赵养学”，李德成’(1)西安市第九医院骨科，陕西西安710054；2)西安交通大学医学院，陕西西安710061)[摘要]目的探讨盐霉素对人骨肉瘤MG一63细胞增殖凋亡的的影响及其分子机制．方法Mrrr法检测盐霉素对人骨肉瘤MG一63细胞增殖的作用，利用流式细胞仪检测盐霉素对MG一63细胞凋亡的作用．Westernblot法检测盐霉素对MG一63细胞中

2、凋亡相关蛋白Bax、Bcl一2及Caspase一3蛋白表达的影响．结果盐霉素可以抑制人骨肉瘤MG一63细胞的增殖，并呈现出时间、剂量依赖效应；经过盐霉素(2M和4IxM)处理的MG一63细胞，与对照组(0M)相比，其凋亡细胞的比率明显升高(P

3、类号]R738．1[文献标识码]A[文章编号]2095-610X(2014)07—0059—04EfectofSalinomycinontheProliferationandApoptosisofHumanOsteosarcomaCellLineMG-63ZHANGJian”，LIBin∞，ZHAOYang—xue”，LIDe-cheng(1)Dept．ofOrthopedics，TheNinthHospitalofXi’an，Xi’帆Shanxi710054；2)SchoolofMedicine，Xi’anJiaotongUniversity，

4、Xi’帆Shanxi710061，China)[Abstract]ObjectiveToinvestigatetheeffectofsalinomycinontheproliferationandapoptosisofhumanosteosarcomacelllineMG一63andtheunderlyingmechanism．MethodsMTTassaywasusedtodeterminetheeffectofsalinomycinontheproliferationofhumanosteosarcomacelllineMG一63．Flowc

5、ytometrywasusedtodetecttheapoptosisrateofMG-63cellundersalinomycintreatment．Theproteinexpressionsofapoptosis—relatedproteinBax．Bcl一2andCaspase一3inMG一63cellsundersalinomycintreatmentwereevaluatedbyWesternblot．ResultsTheresultsshowedthatthetreatmentwithsalinomycinsuppressedtheg

6、rowthofMG-63cellsinatimeandconcentrationdependentmanner；theapoptosisrateofMG一63cellsundersalinomycin(2M，4M)treatmentwasremarkablyincreasedascomparedwiththatinthecontrolgroup(0p．M)．Inaddition，Westernblotresultsshowedthattheexpressionsofpro-apoptosisproteinsBaxandCaspase——3were

7、significantlyup——regulatedundersalinomycin(2p．M，4tzM)treatment(P<0．05)；andtheanti-apoptosisproteinBcl-2wasdown-regulated(P<0．05)．ConclusionSalinomycincaninhibittheproliferationandinduceapoptosisofhumanosteosarcomacelllineMG一63，whichmayberelatedtoregulatingtheapoptosis-related

8、proteinexpressions．[Keywords]Osteosarcoma；Salinomycin；Cellproliferat