学术报告-英文ppt-内容：寡糖的分离和结构鉴定.ppt

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1、StructuralconfirmationofoligosaccharidesnewlyisolatedfromsugerbeetmolassesOutlinesBackgroundAchievementExperimentalConclusion&DiscussionBackgroundWhyshouldwedothisexperi-ment?raffinose;otheroligosaccharides;examinedindetailWhatcontributionscouldthestudybring?newoligosaccharides;structureco

2、mfirmed;fullassignmentofthe1H,13Csignals;2D-NMRFouroligosaccharideswereisolatedThestructuralofthemwereconfirmedAchievement1stThefirstfullassignmentof1H，13Cand2D-NMRofsaccharide1~4werecarriedout2ndExperimentalDilutedfivetimeswithwaterMOLASSESPreparativeHPLCODScolumn35℃water3.5mL/min60timesDIL

3、UTEDMOLASSESMeasuredwithaRefractiveIndexDetectorConcentratedinvacuothenfreeze-driedELUTEDSACCHARIDESPOWDEREDFRACTIONSItoXIIExperimentalbyHPAECandTOF-MSanalysesFractionsItoXIIFractionsXI(13.8mg)FractionsIV(51.6mg)FractionsIX(240.0mg)FractionsII(41.1mg)Experimentalcarbon-Celitecolumn10%ethanol

4、(1.8–2.4L)FractionFractionIVIXFractionIISACCHARIDE1carbon-Celitecolumn5%ethanol(0.3-1.1L)carbon-Celitecolumn10%ethanol(2.5–2.9L)SACCHARIDE2SACCHARIDE3PreparativeHPLCrepeated60timesrententiontime34.5-35.5FractionXISACCHARIDE4Experimental1st2nd3rdHPAECSaccharide1~4wereshowntobehomogene-ous,but

5、didn'tcorrespondtoanyauthenticsaccharidesTOF-MSThepolymerizationwasestablishedas3AcidHydrolyzates→HPLCSaccharides1&2:Glucose,Fructose,Galactose(1:1:1)3&4:Glucose,Fructose(2:1)Methylationandmethanolysis→GLCMethylationwascarriedoutbyHakomorimethodMethanolyzedbyheatingat96℃for30minwith1.5%metha

6、nolichydrochloricacidresultsaccharide31-Kestose1.101.321-Kestose1.471.06Raffinose2.633.741.453.681.291.082.55Methyl-1,3,4,6-tetra-O-methyl-D-fructosideMethyl-2,3,4,6-tetra-O-methyl-D-glucosideMethyl-2,3,4-tri-O-methyl-D-glucosideα-D-glucopyranosyl-(1->6)-α-D-glucopyranosyl-(1<->2)-β-D-fructo

7、furanoside1.08ExperimentalGlcanomericproton→H-1(δH4.98)methyleneproton→H-6(δC61.25)exhibitedcorrelationpeakstobothH-1andH-2byHMBC→C-3(δC73.8)gavecorrelationpeaktoH-1byCOSY→H-2(δH3.57)exhibitedacorrelationpeaktoH-1byHMBC→C-5(δC72.63)Theremainingmeth