氨基酸色谱

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1、EZ:faast™AminoAcidAnalysisTheoryandpracticaloverviewPresentationOutline•Whyanalyze?•OlderTechniquesandLimitations•EZ:faastadvantages•EZ:faastproductoverview•EZ:faastapplications•ConclusionWhyAnalyze?•Whyanalyzeaminoacids?–Determinationofmetabolicdisorders–Fermentation/cellcultureanalysis–Nut

2、ritionallabeling/feedanalysis–Qualitycontroloffoods/beverage–Proteinidentification/characterization–PeptidequalitycontrolOlderTechniquesHistoricalBackgroundandMethodLimitationsOlderTechniques•Pastadvancements–IEXwithpost-columnderivatizationintroducedin1950s–Achievementsmadein•Derivatization

3、&detection•Separationscience(chromatographymedia)OlderTechniques•Derivatizationadvancements–Pre-Columnderivitizationincreasesensitivity–AttachmentofchromaphoreallowsforUVorflorescencedetectionOlderTechniques•Separationscienceadvancements–Chromatographymediaimprovementsincreasedresolu

4、tion•Ionexchangemedias(post-columnderivatization)•Reversedphasemedias(pre-columnderivatization)OlderTechniques•Ionexchangewithpost-columnderivatization–Derivatizingagents•NinhydrinorOPA–Products•Beckman®6300,Pickering®Labs,Hitachi®L880,Biochrom®30series–Strengths•Establishedmethods•Chemistry

5、wellcharacterizedOlderTechniques•Reversedphasewithpre-columnderivatization–Derivatizingagents•ACQ,OPA,PITC,FMOC–Products•Waters®Pico-Tag®&AccQ-Tag™–Strengths•Fasteranalysistimes/improvedthroughput•MoresensitiveOlderTechniques•Ionchromatographywithelectrochemicaldetection–Products•Dio

6、nex®AAADirect®–Strengths•Noderivatizationnecessary•CompatiblewithgelhydrolysisOlderTechniques•Despiteadvancements–Methodlimitations•Samplepreparation•Derivatization•Analysistime/throughput•Cost–Performancelimitations•Resolution/dynamicrange•Accuracy/reproducibility•LimitsofdetectionOlderTech

7、niques•MethodlimitationsIEXwithRPwithICwithpost-columnpre-columnECDdetectionderivatizationderivatizationSamplePreparationComplexprotein&DesaltingrequiredRemovesalts&detergentremovalpriorderivatiza