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1、Articlepubs.acs.org/JAFCWater-CompatibleMolecularlyImprintedMicrospheresinPipetteTipSolid-PhaseExtractionforSimultaneousDeterminationofFiveFluoroquinolonesinEggsSutingLiu,HongyuanYan,MingyuWang,andLihuiWangKeyLaboratoryofPharmaceuticalQualityControlofHebeiProvince&CollegeofPharma
2、cy,HebeiUniversity,Baoding,071002,ChinaABSTRACT:Newwater-compatiblemolecularlyimprintedmicrospheresweresynthesizedbyreversibleaddition−fragmentationchaintransfer(RAFT)polymerizationusing3-(2-carboxyethylsulfanylthiocarbonyl-sulfanyl)propionicacidasahydrophilicchain-transferagent,
3、andemployedasthesorbentofpipettipmolecularlyimprintedsolid-phaseextraction(PT-MISPE)forrapidextractionandscreeningofofloxacin,pefloxacin,norfloxacin,ciprofloxacin,andenrofloxacinineggs.IncomparisontoconventionalSPEmethods,thepresentedPT-MISPEshowedspecialselectivity,easyoperation,anda
4、ccessibledevicewithoutexpensiveSPEapparatus.ThepresentedPT-MISPEmethodcombinedadvantagesofdummymolecularlyimprintedpolymersandpipettipsolid-phaseextraction.Thepresentedmethodwaslinearoveracalibrationrangeof25−2500μg/kgwiththelimitsofdetectionsof0.53−1.07μg/kg.Goodrecoveries(89.1−
5、102.5%)wereachievedwithrelativestandarddeviationsof2.6−4.8%.KEYWORDS:dummymolecularlyimprintedmicrospheres,RAFTpolymerization,pipettetipsolid-phaseextraction,fluoroquinolones,egg16−18■INTRODUCTIONconventionalSPEcartridges.Thesmallcapacityofpipettipwithlesssorbentwouldsignificantlyr
6、educesolventFluoroquinolones(FQs),animportantgroupofsyntheticconsumptioninwashingandelutionsteps,shortentheantibacterialagents,areeffectiveincontrollingawiderangeof171,2operationtimeofSPE,andincreasesamplethroughout.Gram-positiveandGram-negativeorganismbacteria.FQsareThePT-SPEmeth
7、odisnowaneffectivemeansforpurification,usedextensivelyintheprophylaxisandtreatmentofvarious3concentration,andisolationofproteinsandpeptidesindiseasesoffood-producinganimals.However,thewideuseofgenomics,proteomics,metabolomics,etc.However,mostFQswouldresultinresiduesinediblepartsofa
8、nimalsandsorbentsofPT-SPEsufferfromlowsel
