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ID:33008908
大小:2.35 MB
页数:57页
时间:2019-02-19
《猕猴小梁切除术后房水引流机制的分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、复旦大学硕士学位论文TheStudyofTheAqueousHumorOutflowWaysPost—。TrabeculectomyinRhesusMonkey———nleroleofconjunctivallymphaticandvenoussysteminaqueousdrainageAbstractPurposeTotestifytherelationshipbetweentheconjunctivalIymphaticandvenousdrainagesystemandaqueoushumoroutf
2、lowwayspost—trabeculectomy.Methodsandmaterials1、Objectsandgroups:6adultrhesusmonkey,4.7kgto9.8kg,12eyesinal1,Randomlychooseoneeyeforsurgeryandtheotherforcontr01.2、Surgery:limbusbasedtrabeculectomywasperformed.Theconjunctivalflapwasmade8mfrom1imbusanda3X3mmh
3、alf-thicknessscleraflapdissectionwasperformedatthe1imbus,slicingabout1×2mmtrabecular,andthendoiridotomy.10/0sutureforthescleraflap,and8/0suturefortheTenon’Scapsularandconjunctira.Surgeryposition:foureyesinsuperiorandtwoininferior—temporalarea.3、Developedinj
4、ectingexperiment:inject0.1~O.3ml1%trypanblueunderflatconjunctivaby30GneedletodisplaytheIymphaticsystem.Inject0.1~0.2ml5%fluoresceinsodiumintoanteriorchambertofollowtheaqueoushumoroutflowpathways.Thesurgerygroupunderwentfluoresceinsodiumandtrypanbluedevelope
5、rexperimentasself-controlbeforesurgery.Andcontrolgroupunderwentthesametimesstainingexperimentsastheothereyesbutwithoutsurgery.Injectingexperimentdays:twicebeforesurgerywithtwoweeksinterval,and5±2、14±2daysthenevery7±2daysaftersurgery.4、Histologicalstudy:sacr
6、ificethemonkeyaccordingtothefilteringcondition.Cuttingconjunctivaintowholemountandstainingby5’-Nase.Andtheunderconjunctivasurgerypositiontissuewasmadeintoparaffinconnectiveslice.stainedbyHE、antiVEGFR-3andantiCD34inordertostudythedistributionof1ymphaticvesse
7、lSandbloodvesselsinsurgeryarea,alSOtodefinesomevesselsappearedaftersurgery.-7.复旦大学硕士学位论文Resultsl、Surgeryresults:2of6eyeshavemildTyndallphenomenaandweredisappeared7daysaftersurgery.Thefilteringblebweresustainedfor5~16daysand12.4±4.3daysinaverageundersplitlam
8、p。andlastedfor5~26.5days.16.2--+9.8dinaveragebyanteriorchamberfluoresceinsodiuminjectingexperiment.Filteringwerelast19.5~122days,49.7±43.5daysonaverageaftersurgery.Thesuturewereabsorbedorbrushedoff26.1
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