PTEN与基质金属蛋白酶在胃癌组织中的表达及意义.doc

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1、PTEN与基质金属蛋白酶在胃癌组织中的表达及意义作者：朱宝和詹文华刘弋马晋平李正荣【摘要】目的：探讨PTEN与基质金属蛋白酶（MMP）在胃癌组织中的表达、相互关系及意义。方法：应用免疫组化SP技术检测80例胃癌组织中PTEN、MMP2和MMP9表达，同时检测20例正常对照组胃粘膜中PTEN表达。结果：胃癌组织中PTEN高表达率35/80（43.8%）显著低于对照组20/20（100.0%）（P＜0.01）；PTEN表达与胃癌分化程度、浸润深度、淋巴结转移和肿瘤分期显著相关；胃癌组织中MMP2、MMP9阳性表达率分别为41/80（51.3%）、29/80（36.3%），与胃

2、癌浸润深度、淋巴结转移和肿瘤分期显著相关；PTEN与MMP2、MMP9表达显著负相关，与病人预后相关，KaplanMeier生存曲线分析显示PTEN高表达者术后累计生存率显著高于低表达者，PTEN高表达者术后3年、5年生存率显著高于低表达者；MMP2、MMP9阳性表达者术后累计生存率显著低于阴性表达者，MMP2、MMP9阳性表达者术后3年、5年生存率显著低于阴性表达者。结论：胃癌组织中PTEN表达显著减少，PTEN与MMP2、MMP9表达显著负相关，PTEN可能通过调控胃癌组织MMP2、MMP9表达，抑制胃癌的浸润和转移，影响病人的预后。【关键词】胃肿瘤・P

3、TEN・基质金属蛋白酶ExpressionofPTENandmatrixmetalloproteinaseandtheirclinicopathologicandprognosticsignificanceinhumangastriccarcinoma【ABSTRACT】Objective:ToinvestigateexpressionofPTEN,MMP2andMMP9inhumangastriccarcinoma,andtoidentifytherelationshipbetweenexpressionofPTENandthatofMMP2andMMP9

4、andtheirclinicopathologicandprognosticsignificance.Methods:ExpressionofPTEN,MMP2andMMP9wasexaminedbySPmethodimmunohistochemistryin80archivalparaffinembeddedgastriccarcinomatissuespecimens,andexpressionofPTENwasalsoexaminedbySPmethodimmunohistochemistryin20normalgastrictissuespecimensforco

5、ntrol.Results:ExpressionofPTENingastriccarcinoma35/80(43.8%)wassignificantlyreducedthanthatinnormalgastrictissues19/20(95.0%).P＜0.01.ExpressionofPTENsignificantlycorrelatedwithtumordifferentiation,depthoftumorinvasion,lymphnodemetastasisandtumorstaging.ExpressionofMMP2andMMP9were41/80(51.

6、3%)and29/80(36.3%)respectively.ExpressionofMMP2andMMP9significantlycorrelatedwithdepthoftumorinvasion,lymphnodemetastasisandtumorstaging.ExpressionofPTENnegativelycorrelatedwiththatofMMP2andMMP9.ExpressionofPTEN,MMP2andMMP9significantly6correlatedwithpatients’prognosis.KaplanMeiersurvival

7、curvesshowedthatcumsurvivalofpatientswithhighlyexpressionofPTENwassignificantlyhigherthanthosewithreducedexpression.Logranktest,P＜0.05.ButcumsurvivalofpatientswithpositivelyexpressionofMMP2andMMP9weresignificantlylowerthanthosewithnegativelyexpression,Logranktest,P＜