咪喹莫特对小鼠哮喘模型气道炎症和stat6表达的影响论文

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1、咪喹莫特对小鼠哮喘模型气道炎症和STAT6表达的影响论文金淑贤，殷凯生，卞涛，陈子庆【关键词】哮喘EffectsofimiquimodonairmationandSTAT6expressioninmousemodelsofasthma【Abstract】AIM:ToobservetheeffectsofaerosolimiquimodontheantigeninducedairmationandtheexpressionsofTh2typechemokinesEotaxin,macrophagederivedchemokine(MDC)a

2、ndthymusandactivationregulatedchemokine(TARC)inmousemodelsofasthma.METHODS:Imiquimodanddexamethasoneinisteredtoimmunizedmicebeforeantigenchallenge.At24hafterthefinalOVAinhalation,theleftsuperiorlungtissuemationberofcellsandclassifythem.Theexpressionsofsignaltransducersand

3、activatorsoftranscription1(STAT1)andsignaltransducersandactivatorsoftranscription6(STAT6)inlungsmunohistochemistryandRNAexpressionsofSTAT1,STAT6,IL4,eotaxinandIFNγinlungsinedbyreversetranscriptasepolymerasechainreaction.RESULTS:①Imiquimodanddexamethasoneattenuatedtheairma

4、tionofasthmaticmice.②Imiquimoddecreasedthetotolcellcounts,EOSandlymphocytecountsinBALf.③TheexpressionsofSTAT1andSTAT6ainlyonthebronchialepithelium.STAT1andSTAT6proteinsandmRNAlevelsincreasedinlungtissuesinasthmagroup.ImiquimodsignificantlyreducedSTAT1andSTAT6proteinandmRN

5、Aexpression.DexamethasonesignificantlyreducedthelevelsofSTAT6proteinandmRNA,butfailedtoinhibittheexpressionofSTAT1.④ImiquimodmodulatedtheTh1/Th2responsebyenhancingTh1cytokineIFNγmRNAandinhibitingtheTh2cytokineIL4mRNAexpressioninlungtissues.CONCLUSION:Imiquimodaerosolinhal

6、ationmayinhibitantigeninducedairmationanddecreasetheoverexpressionofSTAT6proteinandmRNAinasthmaticmousebuthasnoinfluenceonSTAT1expression.【Keya;signaltransducersandactivatoroftranscription1;signaltransducersandactivatoroftranscription6;imiquimod【摘要】目的：观察咪喹莫特对小鼠哮喘模型气道炎症和肺组

7、织信号转导与转录激活因子1（STAT1）和STAT6表达的影响.方法：建立哮喘模型，自14d时雾化吸入OVA前0.5h，干预组分别雾化吸入咪喹莫特30min及ip地塞米松.OVA雾化结束后24h取左上叶肺组织做HE染色观察肺组织炎症改变；收集肺泡灌洗液进行细胞计数和分类；用免疫组化和ann1表达，促进炎性细胞从血管内募集到炎症部位.咪喹莫特是一种免疫调节剂，它由单核细胞、巨噬细胞、B细胞和树突状细胞等抗原递呈细胞识别，通过与这些细胞表面受体如Tollreceptor7(TLR7)结合，诱导产生前炎症细胞因子IFNα,TNFα和IL12，这

8、些局部产生的细胞因子促使Th1反应优势的产生［7］.体外实验证实它还能促进培养的T淋巴细胞产生IL12和IFNγ，抑制IL4和IL5［4］，使原始Th细胞向Th1细胞分化，提示咪喹莫特可能对治