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1、口腔癌患者外周血树突状细胞的生物学特性姓名，2014年6月25日作者：张素欣，王士杰，单保恩，段玉芹，许艳枝【关键词】U腔肿瘤;树突状细胞;表型；增殖Biologicalcharacteristicsofmonocytederiveddendriticcellsfromperipheralbloodofpatientswithoralcarcinoma【Abstract】AIM:Toinvestigatethemorphology，phenotypeandfunctionsofdendriticce
2、ll(DC)fromperipheralbloodofthehealthyindividualsandthepatientswithoralcarcinoma.METHODS:FifteenpatientswithoralcarcinomaatstagesofIIorabovewereassignedtostudygroupand15healthyvolunteerstocontrolgroup.Monocyteswereisolatedbydensitycentrifugation，andcult
3、uredinthepresenceofGMCSF，IL4andTNFafor7dinvitro.Themorphologicalchangeoftheculturedcellswasobservedbylightmicroscopyandelectronmicroscopy.TheexpressionsofCD83,CDla,CD86,CD80,HLADRwereanalyzedbyflowcytometry.TheabilityofDCtostimulatetheproliferationofly
4、mphocyteinmixedlymphocytereactions(MLR)wasdeterminedwiththemethodofMTT.RESULTS:MorphologicallyandphenotypicallytypicalDCwasobtainedinthe2groupsafterculturefor7d.TheexpressionratesofCD83,CDlaonDCfromthepatientswere49.3士9.5and48.1士7.3respectively,signifi
5、cantlylowerthanthosefromhealthyvolunteers(P0.05).TheDCfromthepatientshadthelowerexpressionlevelsofCD86，CD80andHLADR(85.5士8.7，83.2士8.1and78.3士5.4)thanhealthyvolunteersdid(89.2土6.7,87.7士7.2and82.3士4.1)，butnotsignificantly(P0.05).Moreover，themeanfluoresce
6、nceintensityofCD83,CDla,CD80,CD86andHLADRexpressedinstudygroupwere4.80士2.13,3.96土1.15,19.63士8.12,12.33士6.75and39.44士7.9,respectively,whichwerelowerbutnotstatisticallydifferentfromthecontrolgroup(P0.05).Inaddition，DCfromthestudygrouphadtheabilitytostimu
7、latetheproliferationofallogeneicandautogenouslymphocytesinvitro.CONCLUSION:MonocytesinperipheralbloodofthepatientswithoralcarcinomacanbeinducedtodifferentiateintomatureDCwiththeabilitytostimulatetheproliferationoflymphocytes,andsotheseDCmightbeusedinim
8、munotherapyfororalcarcinoma.【Keywords】mouthneoplasms;dendriticcell;phenotyping;proliferation【摘要】目的：研究口腔癌患者外周血树突状细胞(DC)的形态、表型和功能性变化及其临床意义.方法：口腔癌患者(实验组)及正常人(对照组)15例外周血单个核细胞经GMCSFJL4及TNFa诱导培养，获取成熟DC，光镜和电镜观察细胞的形态，流式细胞仪检测细胞表型如CDla，CD83，CD80，
