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1、-华中科技大学硕士学位论文StudiesonfeasibilityofdetectionofToxoplasmagondiicirculationantigensusingIgYagainstT.gondiisolubleantigenCandidate:ZhouYanSuperviser:Prof.ShenDingwenProf.LiYonglongDepartmentofPathogenicBiology,TongjiMedicalCollege,HuaZhongUniversityofScienceandTechnologyABSTRACTToxopla
2、smosisisazoonosescausedbyT.gondii,Itdistributeswidelyallovertheworld.Theharmoftoxoplasmosistofetushasbeenrecognizedbyclinicalmedicalworkers.WhenpregnantwomenwereinfectedT.gondii,itcanbetransmittedverticallythroughtheplacenta,causingabortion,stillbirth,teratosis,mentalretardation,con
3、genitaltoxoplasmosis,etc.Inrecentyears,withincreasingdeathreportcausedbyT.gondiiofpatientssufferedbyAIDS,cancerandotherimmunefunctiondamage,itwastakengraduallymoreandmoreconcern.Therefore,earlydiagnosisoftoxoplasmosishasgreatsignificancetopreventabnormalfetusandreduceminimumtheharmo
4、fpregnantwomeninfectedT.gondii.Atpresentclinicalcommonmethodstodiagnosetoxoplasmosisincludeetiologicdiagnosis,immunologydiagnosisandmolecularbiology,etc.Etiologycultivationandanimalvaccinationarecomplicatedandthepositiveratewasnotveryhigh.Themethodofmolecularbiologydemandsstrictlabo
5、ratoryconditionsandspecializedtechnician.Detectionofcorrespondingantibodywithserologicalmethods，althoughtheoperationissimple,goodspecificityandsensitivity,itisimpossibletodistinguishcurrentinfectionandpreviousinfection.Soitwasanurgentassignmenttodevelopa4------华中科技大学硕士学位论文techniquew
6、hichcandetectthecirculatingantigenforidentificationofcurrentinfection.Itwasoneoftheprimaryproblemsforresearchworkershowtoselectthehighsensitivityandspecificitydetectionantibody.IthasbeenconfirmedthatIgYantibodyhascharacteristicofantibodyoutputhighly,nocrossreactionwithhuman’scomplem
7、ents,preparationandpurificationsimple,naturestability.InordertoexploretheappliedvalueofIgYindiagnosisofT.gondii,thesolubleantigenofT.gondiiwaspreparedbythephysicalfilteringmethod.ChickenwasimmunizedwithT.gondiisolubleantigen.Specificeggyolkimmunoglobulin(IgY)againstsolubleantigenofT
8、.gondiiwasprepareda
