山苍子无性繁殖技术的.研究

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1、福建农转大学2008届硕士学位论文Usingcompletelyrandomizedexperimentaldesign,SanmingprovenanceoftheShootbrancheshavethehi曲estlaunchrate(81．02％)andProliferationrate(3．06)．Sanmingprovenancewastheoptimal，JiangleprovenanceWasthesecondoneandShaxianprovenanceoftheworstlrain．differentbranchespartsoftheexplants

2、havethedifferentlaunchrateandtheproliferationrate，theshoothavethehighestlaunchrateandtheproliferation，andthenfromtoptobottom，thelaunchrateandtheproliferationratehasdecreased．1．5Effectofcontaminationrate、launchrateandproliferationondifferentmonthsofLitseacubebaCollectedindifferentmonths，t

3、hepollutionrateandtherateofproliferationactivateddifferent,SeptemberandNovemberWaSabeRertimeinthesecondhalfofthetrainingtime，March,AprilSabettertimeinthefirsthalfofthetrainingtime，withthehigherlaunchrateandproliferation．1．6ThesubcultureofLitseacubebaUsingoahogonaldesign，andselectedthefir

4、stsubculturemediumforthebestWasMS+I．0mg／LBA+0．5mg／LKT+0．4mg／LIBA+309／LSucrose．thesecondsubculturemediumWasMS+I．Omg／LBA+0．1mg／LKT+0．4mg／LIBA+309／LSucrose+O．59／LAC．thethirdsubculturemediumwasMS+0．5mg／LBA+0．2mg／LIBA+209／LSucrose+o．59eLAC．1．7TherootingcultureandtransplantingofLitseacubebaUsi

5、ngorthogonaldesign，，takingintotheeffectofdifferenthormonesontherootingofLitseaCubeba,selectedthebestmediumoftherootingculturewasI／2MS+0．Smg／LIBA+0．2mgCLIAA+209／LSucrose．TransplantingmediaWasthesandinthefirst，andthentransplanttothemedia(sand：yellowsoil：peatsoil=1：1：1)later．2．Callusinducti

6、on,subcultureandregenerationofLitseacubebaUsingthreedifferentexplants(stems，leaves，petioles)toinducethecallus，andestablishedcallussystemofLi括eaCubeba．Differentexplants(stems，leaves，petioles)haddifferentdifficultytoinducethecallus：stems>petioles>leaves．stemswerethebestmaterialofa11．Theopt

7、imummedisofstemcallusinductionwasMS+2．Omg／t,2,4-D+1．Omg／LBA+I．0mg／LKT+309／LSucrose．TheoptimummedisofleavecallusinductionWasMS+I．5mg／L2,4-D+I．0mg／LBA+0．5mg／LKT+309／LSucrose．TheoptimummedisofpetiolescallusinductionwasMS+I。5mg／L2,4-D+1。OmgCt,BA+I．0mg／LKT+309／LSucrose．Thebest