中药两头尖的质量控制与其毒性分析

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1、摘要竹节香附素A峰计算应不低于4000。1l批次两头尖样品共确定8个共有峰，传统夹角余弦相似度cos9均在O．97以上，符合建立指纹图谱的要求。cos8’在0。115和0．951之间变化，Ⅳ在0．045和1．129之间变化，表明cos0’和Ⅳ可以更显著的区分样品。样品S9的COS目’和H值最低，分别为0．115和0．045；S3的值最高，分别为O．951和1．129。FT-IR指纹图谱，扫描范围为1500—800cm～，样品S5的cos0’和日值最高，S8最低。uv指纹图谱，扫描范围为210．400nm，样品S6的cosO

2、’和日值最高，sll最低。采用SAS统计软件对样品进行聚类分析，s4和s11首先被聚为一类；其次为S1和s6。S8与其它的样品距离最远。两头尖水提物小鼠灌胃毒性实验LD50及其95％可信限分别为104．50和95．45—115．29g生药／l(g。按照Blachwell法推算相当于10．45g／L，说明在临床应用常用量为1．3g一般是安全的。按照中药毒性分级，两头尖介于“中毒”和“小毒”之间。石油醚、氯仿和正丁醇萃取部位小鼠最大耐受量实验，灌胃给药1000g生药／kg,14天后小鼠未出现明显中毒症状，小鼠体重也无明显改变，

3、与对照组比较无统计学差异。解剖后，肉眼观察其主要脏器，未发现明显异常，说明分别口服两头矢石油醚、氯仿和正丁醇萃取物1000g／kg，对小鼠都无明显急性毒性作用：乙酸乙酯莘取部位的LD50及95％的可信限分别为604．81和537．99．673．57g生药／kg。关键字：两头尖，质量控制，指纹图谱，毒性IIlHangzhouNormalUniversity1hesisforMaterAbslractAbstractTheRhizomeofAnemoneraddeanaRegelisakindoftraditionalChin

4、esemedicine(TCM)．Traditionally，itisusedtotreatneuralgiaandrheumatism．Recently,manytriterpenoidsaponinshavebeenseparatedanddeterminedfromA．raddeanaRhizome，whichcouldberesponsibleformanybioactivitiesofA．suchasantiinflammation，antitumor,antieclampsia，andantipyretican

5、danalgesia．ButthequalitycontrolofA．raddeanaRhizomehasnotbeenexaminedandthetoxiccompoundsandtoxicologyofA．raddeanaRhizomehavenotbeendeterminedeither．ThisthesisreportedthefirststudytothequalityevaluationandacutetoxicityofA。raddeanaRhizomeinordertopromotethedevelopme

6、ntofitInthisthesis，thinlayerchromatography(TLC)wasthefirstmethodofchoiceforherbalanalysis，andwasusedforevaluationofthequalityof1batchsamplesofA，raddeanaRhizomecollectedfromnortheastChina．Highperformanceliquidchromatography(HPLC)wasemployedtodeterminethecontentsofR

7、addeaninAin1Isamples．HPI,C，FT-IR(Fourier-transforminfraredspectroscopy)andUV(Ultraviolet)methodswereappliedtoestablishthefingerprintsofA．raddeanaRhizomeforqualityevaluation．Beforeconstructingthefingerprint，theoriginaldatawasstandardizedinordertoeliminatetheinfluen

8、ceofthedimension．Thenthestandardizeddatawasusedtocalculatethecosineofunitvectorcos0．Inordertocomprehensivelydescribethedifferencebetweenthesamplechromat