重楼近缘种及商品药材的dna条形码鉴定研究

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1、泸州医学院硕士学位论文中图分类号：R89；R36；R39硕士学位论文重楼近缘种及商品药材的DNA条形码鉴定研究院（系）、所药学院研究生姓名姜黎学科、专业中药学导师姓名孙琴副教授二Ο一三年四月1泸州医学院硕士学位论文目录摘要..................................................................................................1Abstract...................................................................

2、...............................21前言.....................................................................................42材料与方法..............................................................................62.1实验材料........................................................................

3、..........62.2实验仪器与试剂......................................................................82.3样品总DNA的提取................................................................92.3.1提取方法..................................................................................92.3.2DNA浓度及纯度的检测........

4、...............................................112.4PCR扩增................................................................................122.5纯化和测序............................................................................132.6数据处理.....................................................

5、...........................152.6.1遗传距离分析........................................................................152.6.2WilcoxonSignedRankTests检验........................................162.6.3系统发育关系分析评价鉴定效率........................................163结果...................................

6、......................................................163.1PCR扩增效率和测序成功率................................................163.2候选片段的序列特征............................................................173.3不同DNA片段种内种间差异分析......................................183.3DNA条形码候选序列的barcodinggap.....

7、..........................193.4两两序列种内种间差异的wilcoxon秩和检验...................233.5系统发育关系分析评价鉴定效率.......................................274讨论........................................................................................295结论........................................................

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