水样中DNA提取方法_整理

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1、ContentsIntroduction®Introduction...........................................2TheE.Z.N.A.WaterDNAKitallowsrapidandreliableisolationofhigh-qualityDNAfromvariousmicroorganismsinwatersamples.ThesystemcombinestheOverview.............................................

2、2®reversiblenucleicacid-bindingpropertiesofHiBindmatrixwiththespeedandversatilityofspincolumntechnologytoeliminatePCRinhibitingStorageandStability.....................................2compoundsfromwatersample.PurifiedDNAissuitableforPCR,restrictionKitContents.

3、..........................................3digestion,andhybridizationtechniques.Therearenoorganicextractionsthusreducingplasticwasteandhands-ontimetoallowmultiplesamplestobeMaterialstoBeProvidedbyUser.............................3processedinparallel.BeforeStar

4、ting.........................................4®E.Z.N.A.WaterDNAKitProtocol............................5OverviewTroubleshootingGuide....................................7®IfusingtheE.Z.N.A.WaterDNAKitforthefirsttime,pleasereadthisbooklettobecomefamiliarwiththepr

5、ocedure.Watersampleisfirstfilteredusingmicroporousfilter.Thefilteristhenputintoatubecontainsbeadsandbuffertohomogenizeandlysisthesample.Humicacid,proteins,polysaccharides,andothercontaminantsaresubsequentlyprecipitatedafteraheat-frozenstep.Contaminantsarefurth

6、erremovedbyaspecialHTRreagenttreatment.Bindingconditionsarethenadjustedandthesampleis®appliedtoanHiBindDNAspin-column.TworapidwashstepsremovetracecontaminantsandpureDNAiselutedinwaterorlowionicstrengthbuffer.PurifiedDNAcanbedirectlyusedindownstreamapplications

7、withouttheneedforfurtherpurification.StorageandStability®ooAllcomponentsoftheE.Z.N.A.WaterDNAKitshouldbestoredat22C-25C.Underthisstorageconditions,DNAhassuccessfullybeenpurifiedandusedforPCRafter24monthsofstorage.Duringshipment,orstorageincoolambientconditions

8、,precipitatesmayforminsomebuffers.Itispossibletodissolveosuchdepositsbyincubationthesolutionat65C.2KitContentsBeforeStarting®ProductNumberD5525-00D5525-01D5525-02!Pleasereadtheenti