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1、BMCBiotechnologyBioMedCentralMethodologyarticleOpenAccessHeatingasarapidpurificationmethodforrecoveringcorrectly-foldedthermotolerantVHandVHHdomainsAurelienOlichon1,DanielSchweizer2,SergeMuyldermans3andAriodeMarco*2,4Address:1CellBiologyUnit,EMBL–Meyerhofstr.1,D-69117,Heidelber
2、g,Germany,2ScientificCoreFacilities,EMBL–Meyerhofstr.1,D-69117,Heidelberg,Germany,3DepartmentofCellularandMolecularInteractions,VIB,VrijeUnversiteitBrussel,Pleinlaan2,B-1050,Brussel,Belgiumand4IFOM-IEOCampus,BiochemistryUnit,viaAdamello16,I-20139,Milano,ItalyEmail:AurelienOlich
3、on-aurelien.olichon@embl.de;DanielSchweizer-daniel.schweizer@embl.de;SergeMuyldermans-svmuylde@vub.ac.be;AriodeMarco*-ario.demarco@ifom-ieo-campus.it*CorrespondingauthorPublished:26January2007Received:15June2006Accepted:26January2007BMCBiotechnology2007,7:7doi:10.1186/1472-6750
4、-7-7Thisarticleisavailablefrom:http://www.biomedcentral.com/1472-6750/7/7©2007Olichonetal;licenseeBioMedCentralLtd.ThisisanOpenAccessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense(http://creativecommons.org/licenses/by/2.0),whichpermitsunrestricteduse,dis
5、tribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.AbstractBackground:RecombinantantibodiesfromCamelidae(VHHs)arepotentiallyusefultoolsforbothbasicresearchandbiotechnologicalapplicationsbecauseoftheirsmallsize,robustness,easyhandlingandpossibilitytorefo
6、ldafterchemio-physicaldenaturation.Theirheattoleranceisaparticularlyinterestingfeaturebecauseithasbeenrecentlyrelatedtobothhighyieldsduringrecombinantexpressionandselectivepurificationoffoldedprotein.Results:PurificationofrecombinantRE3VHHbyheattreatmentyieldedthesameamountofan
7、tibodyaspurificationbyaffinitychromatographyandnegligibledifferenceswerefoundinstability,secondarystructureandfunctionality.Similarresultswereobtainedusinganotherclassofthermotolerantproteins,thesingledomainVHscaffold,describedbyJespersetal.[8].However,thermosensitiveVHscouldno
8、twithstandtheheattreatmentandco-precipitatedwiththebac
