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1、兔耳增生性瘢痕模型的建立及动态观察1111111122胡启擂蔡晓敏娄露萍卢妮娜顾琳媛黄蓉钱丽娜相嘉慧宋跃华胡章颜(1绍兴文理学院医学院检验06级，2绍兴文理学院医学院人解教研室浙江绍兴312000)[摘要]：目的：通过兔耳瘢痕模型的制作和组织学技术，动态观察瘢痕形成的过程、瘢痕组织与正常组织的区别，探讨增生性瘢痕形成的变化规律。方法：通过损伤新西兰大耳白兔耳内侧皮肤，损伤后7天去痂；建立起增生性瘢痕的动物模型；运用常规HE染色和免疫组化技术，进行3个月的大体形态和组织病理观察。结果：兔耳损伤创面在经过早期的炎症反应和肉芽组织增生后，逐渐上皮化；在术后3O天时，形成了色红，质硬，突
2、出皮面的瘢痕；并在术后2个月内持续增生，3个月时仍维持其硬度厚度和颜色。3O天的瘢痕组织学显示：组织水肿，淋巴细胞浸润，微血管增生，成纤维细胞大量增生，排列紊乱；胶原纤维增生，排列紊乱，局部有涡状及结节状排列；网状纤维增多；弹力纤维缺失。术后2个月和3个月时，胶原束渐粗大，成纤维细胞数减少，炎症反应消失。结论：创面形成后出现红肿、结痂，约1个月后瘢痕形成，HE染色和免疫组化可见瘢痕组织真皮增厚，细胞形态改变，成纤维细胞增生明显，与瘢痕的形成密切相关。α-平滑肌肌动蛋白呈不规则分布，对瘢痕增生及挛缩有重要作用。[关键词]：增生性瘢痕；成纤维细胞；α-平滑肌肌动蛋白；动物模型中图分类
3、号：文献标识码：EstablishmentandDynamicInvestigationofrabbitshypertrophicscarsmodelHuqi-lei,Caixiao-ming,Loulu-ping,Luni-na,Guling-yuan,Huangrong,Qianli-na,Xiangjia-hui,Songyue-huaMedicalCollageofShaoxingUniversity,Shaoxing312000,China[Abstract]：Objective：Toestablishanddynamicinvestigateanimalmodelsf
4、orhypertrophicscarsthoughcreatingwoundsovertheventralsurfaceoftherabbitsears．Methods：woundswerecreatedovertheventralsurfaceoftherabbitsears.on7thdaypostwounding,theeschawasremoved.Thehistologicalchangeswereobservedfor3monthsbyHEstainandimmunohistochemistry.Results：Aftertheinflammation,theform
5、ationofthegranulationtissue,theulceratedareaepithelizedstepbystep,On30thdaypostwoundingVisiblyraisedandpalpablescarsappeared,Eachofthescarsremainedraisingin60daysorbeyond,andremainedtheshapeon90thdaypostwounding.Histologically,thescarson30thdaywerenotedtobethickenedcomparedwithsurroundingtiss
6、ue.Thescarsweredemonstratedlargelyhorizontallyarrangedcollagenlbers，increasedvasculartissueandmildchronicinflammation．Theelevatedareaofthescarshadirregulararrangedcollagenfibers，oftenwithacircularorwhirledappearance．Therewerealsoincreasedvaseularityandproliferatedfibmblastson60thand90thday，th
7、escarsremainedirregularlyarrangedcollagenfadersandfibmblasts.Conclusion：Woundsaftertheformationofflare,scab,aboutamonthaftertheformationofhypertrophicscar,scartissuethickeningofthedermis,thecellstochangeshape,fibroblastproliferationsignifican
