Evaluation of therapeutic efficacy of capecitabine on human breast

《Evaluation of therapeutic efficacy of capecitabine on human breast》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、Biomedicine&Pharmacotherapy61(2007)553e557www.elsevier.com/locate/biophaEvaluationoftherapeuticefficacyofcapecitabineonhumanbreastcarcinomatissuesandcelllinesinvitroWingsT.Y.Looa,b,HironobuSasanoa,LouisW.C.Chowb,*aDepartmentofPathology,TohokuUniversitySchoolof

2、Medicine,Sendai,JapanbUNIMEDMedicalInstitute,Wanchai,HongKongAvailableonline14September2007AbstractBackground:Capecitabine,anoralanticancerprodrug,wasassociatedwithrelativelymilddegreesofsideeffects,notablylowmyelosuppres-sion.Previousstudiesdemonstratedcapec

3、itabinemonotherapyassafeandveryusefulforrecurrentandadvancedmetastaticbreastcancerpa-tientswhoaregenerallyassociatedwithapoorprognosis.Materialsandmethods:Capecitabineandtwoconventionalcytotoxicantineoplasticdrugs(epirubicinanddocetaxel)wereemployedinthisstud

4、yinordertocomparetheirtherapeuticantineoplasticeffects.Thetumourtissuesobtainedfrom42patientsandbreastcarcinomacelllinesweretreatedwiththechemotherapeuticagentsaboveinvitro.TheirefficacywasdeterminedusingATPBioluminescenceassaywhichmeasuresthemetabolicrate,WST

5、-1assaythatcouldquantifytheinhibitionofcellproliferationandimmunohistochemicalstudies.Theproapoptoticef-fectswasexaminedusinginsituapoptosiskit.Results:Bothsolidtumourandcelllinestreatedbycapecitabineaswellastwodrugsabovedemonstratedasignificantdecrementinmeta

6、bolicandproliferationrates.However,capecitabinetreatmentresultedinsignificantincrementofthenumberofthetumourcellsundergoingapoptosis.Conclusion:Resultsofourpresentstudydemonstratedthatcapecitabinecouldbeausefulagentagainstbreastcancercellswithlesssideeffectsan

7、ditsinhibitionofcellproliferationofbreastcarcinomaisatleastsimilartothatoftwoothercommonlyusedcytotoxicdrugs.Ó2007ElsevierMassonSAS.Allrightsreserved.Keywords:Capecitabine;Monotherapy;Prodrug;Cytotoxicdrugs;Apoptosis1.Introductionthatcapecitabineresultedinare

8、sponserateof20%,witharel-ativelyimpressive29%responserateinaretrospectivelyde-Capecitabine,athymidinephosphorylase(TP)-activatedfinedsubpopulationofthepatientsrefractorytobothfluoropyrimidi