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1、AdvancePublicationbyJ-STAGEJapaneseJournalofInfectiousDiseasesAnultra-rapidreal-timeRT-PCRmethodfordetectingMiddleEastrespiratorysyndromecoronavirususingamobilePCRdevice,PCR1100KazuyaShirato,NaganoriNao,ShutokuMatsuyama,andTsutomuKageyamaReceived:October10,2019.Accepte
2、d:November21,2019Publishedonline:December25,2019DOI:10.7883/yoken.JJID.2019.400AdvancePublicationarticleshavebeenacceptedbyJJIDbuthavenotbeencopyeditedorformattedforpublication.OriginalArticleAnultra-rapidreal-timeRT-PCRmethodfordetectingMiddleEastrespiratorysyndromeco
3、ronavirususingamobilePCRdevice,PCR11001KazuyaShirato,1NaganoriNao,1ShutokuMatsuyama,and2TsutomuKageyama1LaboratoryofAcuteRespiratoryViralDiseasesandCytokines,DepartmentofVirologyIII,and2InfluenzaVirusResearchCenter,NationalInstituteofInfectiousDisease,4-7-1Gakuen,Musas
4、himurayama,Tokyo208-0011,JapanRunningtitle:Ultra-rapidreal-timeRT-PCRforMERS-CoVKeywords:MiddleEastRespiratorySyndrome(MERS),MERScoronavirus(MERS-CoV),ultra-rapidreal-timeRT-PCR,PCR1100Addresscorrespondenceto:KazuyaShirato,DVM,PhD.SeniorResearcherDepartmentofVirologyII
5、INationalInstituteofInfectiousDiseases,MurayamaBranch4-7-1Gakuen,MusashimurayamaTokyo,208-0011,JapanE-mail:shirato@nih.go.jpTel:+81-42-561-0771Fax:+81-42-567-5631AcceptedManuscript1著者一覧白戸憲也〒208-0011東京都武蔵村山市学園4-7-1国立感染症研究所ウイルス第3部直亨則〒208-0011東京都武蔵村山市学園4-7-1国立感染症研究所ウイルス第3
6、部第4室松山州徳〒208-0011東京都武蔵村山市学園4-7-1国立感染症研究所ウイルス第3部第4室影山努〒208-0011東京都武蔵村山市学園4-7-1国立感染症研究所インフルエンザウイルス研究センター第2室責任著者連絡先白戸憲也〒208-0011東京都武蔵村山市学園4-7-1国立感染症研究所ウイルス第3部Tel:042-561-0771AcceptedManuscriptFax:042-567-5631E-mail:shirato@nih.go.jp2SummaryMiddleEastrespiratorysyndrome(ME
7、RS)coronavirus(MERS-CoV)isusuallydiagnosedthroughhighlysensitiveandspecificgenetictestssuchasreal-timereversetranscriptionpolymerasechainreaction(RT-PCR).Currently,tworeal-timeRT-PCRassaystargetingtheupEandORF1aregionsoftheMERS-CoVgenomearewidelyusedandarethestandardas
8、saysrecommendedbytheWorldHealthOrganization(WHO).TheMERSoutbreakstodatesuggestthatrapiddiagnosisandsubsequentisolatio
