电刺激大鼠皮层桶状区对胆碱乙酰移位酶表达的影响.pdf

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1、312ChinJApplPhysiol，2013，29(4电刺激大鼠皮层桶状区对胆碱乙酰移位酶表达的影响樊红琨，杨春¨，张艳燕，乐小平2，郑春光，师黎，章茜(1．郑州大学基础医学院生理学教研室，2．组织学与胚胎学教研室，3．电气工程学院，河南郑州450001)【摘要】目的：观察电脉冲刺激大鼠大脑皮层桶状区(BC)诱导的转向运动，并观察电刺激后胆碱乙酰移位酶(CHAT)在大鼠Bc区的表达。方法：将大鼠随机分为3组：空白组、对照组、实验组。对照组和实验组大鼠在双侧Bc区埋植刺激电极；术后7d实验组大鼠给予电刺激诱导左右转向

2、运动；连续刺激3d后，取脑行冰冻切片常规HE染色和免疫组织化学染色；并提取3组大鼠海马和脑组织蛋白，用Westernblot方法检测ChAT的表达。结果：给予电脉冲刺激大鼠Bc区，实验动物均出现向左或向右转；HE染色显示电极周围组织没有明显形态学变化。与对照组和空白组相比，免疫组化和Westernblot结果均显示实验组大鼠BC区ChAT阳性的表达率增高。结论：电刺激BC区可诱导大鼠产生特定的方向性运动；且大鼠脑组织中ChAT表达增加，提示胆碱能神经递质参与了感觉．运动皮层之间的信息传递。【关键词】大脑皮层桶状区；电刺

3、激；胆碱乙酰移位酶；乙酰胆碱【中图分类号】R338．2【文献标识码】A【文章编号】1000．6834(2013)04-312—05Expressionofcholineacetyltransferaseintheratbarrelc0rtexbyelectricalstimulationFANHong-kuIl，YANGChun1，ZHANGYah．yan1，LEXiao--pir~2，ZI-I~GChtm-~，sⅧL，ZHANGQian△(1．Department0fPhysiology，MedicalSchool，

4、ZhengzhouUniversity，2．DepartmentofHistologyandEmbryology，3．SchoolofElectricalEngineering，Zh~zho450001，aliIIa)【AKC．lIIACT】Objective：Toobserveaturningperformanceintheratsexcitedbyu~ingaproperelectricalstimuliofthebarrdcortexregion(BC)，andtheexpressionofcholineacet

5、yllransferase()inBCregionsafterelectoralstimulation．Methods：SDratswel'edividedintothreegroups．Thestimulationelectrodesw眦surgicallyimplantedintothebilateralBCregionsinthecontrolgroupandtheexperimentalgrouprats．Theexperimentgrouppostsurgeryfor8evenlyswasgiventheel

6、ectricalimpulsesv／aconnectionwiththeelectrodesforthreetimeseachdaytl】consecutivethreedays．rI11reegroupsoftheratsw骶killedandthebrainswerequicklyremovedforfrozensectionsandthenperformedwithconventionalHEandimmtmohistochemistrystaining．Andproteinsampleswereq)日]redf

7、rombrainandthehippocampustis—sacsofthethreegroupstodetecttheleveloftheChATproteinbyWesternblot．Results：TheexperimentalratsturnleftOrrightwhencontinu·ouslystimulationinthebilateralBCregionswithelectricpulse．HEstainingshowednosignificantdamagearoundelectrodesinthe

8、cerebralcortex．Comparedwiththecontrolandblankgroups，theChATpositiverateinthebrainsectionintheexperimentalratswassignificantlyhighbyimmunohistoehemistryassay；thelevelo