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1、BioresourceTechnology101(2010)7789–7796ContentslistsavailableatScienceDirectBioresourceTechnologyjournalhomepage:www.elsevier.com/locate/biortechExtremethermophilicbiohydrogenproductionfromwheatstrawhydrolysateusingmixedculturefermentation:Effectofreactorconfigurati
2、onPrawitKongjan,IriniAngelidaki*DepartmentofEnvironmentalEngineering,TechnicalUniversityofDenmark,DK-2800Lyngby,DenmarkarticleinfoabstractArticlehistory:Hydrogenproductionfromhemicellulose-richwheatstrawhydrolysatewasinvestigatedincontinu-Received13January2010ously
3、-stirredtankreactor(CSTR),up-flowanaerobicsludgebed(UASB)reactor,andanaerobicfilterReceivedinrevisedform28April2010(AF)reactor.TheCSTRwasoperatedatanhydraulicretentiontime(HRT)of3days,andtheUASBandAccepted6May2010AFreactorswereoperatedat1dayHRT,usingmixedextremetherm
4、ophilesat70°C.Thehighesthydro-genproductionyieldof212.0±6.6mL-H2/g-sugars,correspondingtoahydrogenproductionrateof821.4±25.5mL-H2/dLwasachievedwiththeUASBreactor.LoweringtheHRTto2.5dayscausedcellKeywords:masswashoutintheCSTR,whiletheUASBandAFreactorsgavefluctuatinga
5、ndreducinghydrogenpro-Hemicellulosesductionata0.5-dayHRT.TheoriginalrateandyieldwererecoveredwhentheHRTwasincreasedbacktoBiohydrogenMixedculture1day.Theseresultsdemonstratethatreactorconfigurationisanimportantfactorforenhancingandsta-ExtremethermophilicbilizingH2pro
6、duction.Ó2010ElsevierLtd.Allrightsreserved.1.Introductiontwomainstreams:acellulose-richfiberfractionandahemicellu-lose-richliquidfraction(hydrolysate)(Thomsenetal.,2008).Anaerobichydrogenproductionfromorganicsubstratesisenvi-Whilefermentationofcelluloseforethanolpro
7、ductionisawell-ronmentallyfriendlyandeconomicallyandtechnicallyfeasibleestablishedprocessandefficientlycarriedoutbySaccharomyces(Hawkesetal.,2007).Mixedculturefermentativebacteriaderivedcerevisiae,wild-typestrainsofthisyeasthavedifficultiesinfromnaturalsourcescanconv
8、ertcarbohydratescontainedinligno-fermentinghemicelluloses(Larsenetal.,2008).Therefore,amixedcelluloses,ororganicwaste,intohydrogen,CO2andsolubleo
