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1、中国水稻科学(ChinJRiceSci),２０１６,３０(３):２６５－２７２http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１８８２６５云南地方水稻品种抗稻瘟病基因Pita功能区段序列变异分析马继琼＃杨奕＃孙一丁王炎炎许明辉∗(云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室,昆明６５０２２３;＃共同第一作者;∗通讯联系人,EＧmail:xuminhui＠sohu．com)SequenceVariationofFunction
2、alFragmentinPitaGeneforRiceBlastResistanceinYunnanRiceLandracesMAJiＧqiong＃,YANGYi＃,SUNYiＧding,WANGYanＧyan,XUMingＧhui∗(InstituteofBiotechnologyandGeneticResources,YAAS,KeyLaboratoryofBiotechnologyResearchofYunnanProvince/KeyLaboratoryofSouthwesternCropGeneResourcesandGermplasmI
3、nnovationofMinistryofAgriculture,Kunming６５０２２３,China;＃Theseauthorscontributedequallytothiswork;∗Correspondingauthor,EＧmail:xuminhui＠sohuc．om)MAJiqiong,YANGYi,SUNYiding,etal．SequencevariationoffunctionalfragmentinPitageneforriceblastresistanceinYunnanricelandraces．ChinJRiceSci,
4、２０１６,３０(３):２６５Ｇ２７２．Abstract:Identificationandanalysisofthevariationsofriceblastresistancegenesequenceisthebasistodigintothegeneticresourcesofthericeblastresistance．Inthispaper,６０９basesinthesecondcodefunctionalregionofthericeblastresistancegenePitawereanalyzedusing２１８ricelandra
5、cesfrom５６countiesofYunnanProvince．Atotalof１８nucleotidevariationswerefoundinthesequenceandtheORFsof２１８riceaccessionsweregroupedinto４６haplotypesand２８proteinstypes．PitaＧH１(１７．８９％),PitaＧH２(２９．８２％)andPitaＧH６(２０．１８％)werehighＧfrequencytypesinYunnan,whileothertypeswereatlowfrequency(０
6、．４６％－３．６７％)．SotheadvantagehaplotypesinYunnanwerePiＧtaＧH１,PitaＧH２andPitaＧH６(totally６７．８９％)．Atotalof６１ricelandracescarryingtheresistancegenePita,thebasefunctionalsite２７５２wasGwiththerateof２７．９８％．ThesequenceofPitain３９resistancevarietieswasidenticalwiththesequenceobtainedfromtheVie
7、tnameseresistancevarietyTetep．ThedistributionofresistancegenePitainricesubspeciesorecotypeswasunbalanced．Thedistributionfrequencyofindicawas３５．０６％,japonica２４．１１％,rice３１?０３％,uplandrice２１．９２％,noＧglutinousrice３０．７７％,glutinousrice１８．３７％．ThedistributionofblastresistancegenePitainYu
8、nnanProvincefeaturedwidedistributionandsmallconcentration．Pit
