玉米rca基因克隆及表达分析

《玉米rca基因克隆及表达分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、玉米RCA基因克隆及表达分析玉米RCA基因克隆及表达分析印志同印志同ztyin@yzu.edu.cn扬州大学农学院玉米遗传育种课题组扬州大学农学院玉米遗传育种课题组•改善作物光合性能是提高产量的重要途径之一•Rubiscoactivase(RCA)catalyzestheactivationofRubiscoinvivoandplaysacrucialroleinregulatingplantgrowth.RCARCA是一种核编码的叶绿体蛋白，具有分子伴侣的功能，在多数物种中含有α和β两种亚基。•Inmaize,twoβ-

2、isoformRCAgenes(Zmrca1andZmrca2)withanidenticalopenreadingframe(ORF)werereported.翻译43kD（β亚基）水解41kDVargas-Suarezetal.,JExpBot,2004Ayala-Ochoaetal.,Biochimie2004玉米在热胁迫条件下产生45-46kDRCA多肽ZoranRisticetal.JExpBot,2009Questions1玉米中是否存在RCAα亚基和基因？2前人报道的45-46kDRCA多肽来源？3玉米RCA

3、基因与产量的关系如何?RESULTS1.GenomicAnalysisandcDNACloningRevealedaNewα-formRCAGeneandaPreviouslyReportedβ-formGene2.Zmrca1andZmrca3EncodedtwoDifferentMaizePolypeptidePanelA:anti-Zmrca3antibody.PanelB:anti-Zmrca1antibody.Lane1:pET-30a-Zmrca1PanelC:nativeZmrca1(lane1),lane2

4、:pET-30a-Zmrca3nativeZmrca3(lane2),lane3:emptyE.colileafprotein(lanes3,4)lane4:E.colitransformedwithemptypET-30alane5:Arabidopsislane6:maize.RecombinantNativeZmrca3Zmrca3RecombinantNativeZmrca1Zmrca1MALDI-TOF-MSanalysis3.Zmrca1andZmrca3WereTargetedtoChloroplast4.Z

5、mrca1andZmrca3ShowedLeaf-predominantandCyclicDay/NightExpressionPatterns5.TheExpressionLevelsoftheZmrca1andZmrca3WereCorrelatedWithGrainYieldinMaizeMarkerlociassociatedwithgeneexpressionandgrainyieldin123maizeinbredlinesCONCLUSION•Inmaize,anewα-isoformgeneZmrca3fu

6、nctionsalongsideapreviouslyreportedβ-isoformgeneZmrca1.•TheZmrca3encodesalargermaizeRCApolypeptide,anditsproteinproductislocalizedinchloroplasts.•SimilartotheZmrca1,theZmrca3transcriptsaccumulatemoreintheleafthaninothertissuesandshowcyclicvariationsduringaday/nigh

7、tperiod.•BothZmrca1andZmrca3playanimportantroleindetermininggrainyieldandmayhavepotentialuseinbreedingapplicationstoenhancemaizeproductivity.Yinetal.,(2013)Anα-isoformgenefunctionsalongsideaβ-isoformgeneinmaize,submittedtoPlantPhysiology.Inrevision近年来以第1作者身份发表的SCI

8、文章1)ExpressionquantitativetraitlocianalysisoftwogenesencodingRubiscoactivaseinsoybean.PlantPhysiology,2010，152:1625-1637（IF>6)2)Mappingofquantitativetra