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1、uPA和uPARmRNA高表达与肝细胞癌的关系?394?生第l3卷第5期ChinJClinOneolRehabil.Oct0ber2006.V0113.N0.5uPA和uPARmRNA高表达与肝细胞癌的关系?临床研究?周奇张亮胡琼珍张福君甄宇洋(1.中山大学第一附属医院黄埔院区外一科,广州510700;2.中山大学附属肿瘤医院影像介入中心,华南肿瘤学国家重点实验室;3.南方医科大学珠江医院层流病房)【摘要】目的检测uPA,uPAR在肝癌组织中的表达并探讨其临床意义.方法用RT—PCR法对肝癌,癌旁,正常组织各27例进
2、行uPA,uPAR检测,并结合临床病理资料进行分析.结果uPA,uPAR在肝癌组织中阳性率分别为70.4%(19/27)和77.8%(21/27);相对表达强度分别为0.964±0.458和0.784±0.322,均较癌旁组织及正常组织高,差异有显着性.阳性患者相对表达强度与肿瘤大小及部分侵袭转移指标中表达呈显着相关.结论uPA,uPAR在肝癌组织及部分侵袭转移指标中表达升高,提示二者可能参与了肝细胞癌的发生及侵袭转移.【关键词】肝肿瘤;uPA;uPAR【中图分类号】R735.7;R730.43【文献标识码】A【文章
3、编号】1005.8664(2006)05-0394-04OverexpressionofuPAanduPARmRNAintissueofhepatocellularcarcinomaanditsclinicalsignifi-CauceZHOUQi,ZHANGLiang,HUQiong—zhen.etal(The1stSurgeryDepartmentofHuangpuBranchoftheFirstAffiliatedHospital,ZhongshanUniversity,Guangzhou510700,Chin
4、a)【Abstract】0bjectiveTodetectthemRNAexpressionofuPAanduPARinsamplesofHCCandadjacenttissueandtoelucidateitsclinicalsignificance.MethodsTwenty—sevenhumanhepatocellularcar-cinomatissuesampleswiththeiradjacenttissuesamplesand27normallivertissuesampleswereexaminedby
5、reversetranscription—polymerasechainraction(RT—PCR).TherelationshipbetweenthemRNAexpressionandtheirchnic0path0l0caldatawereanalyzed.ResultsTheexpressionratesofuPAanduPARinhepato—cellularcarcinomatissuesampleswere70.4%(19/27)and77.8%(21/27)respectivelyandtherela
6、tiveex—pressionintensityofuPAanduPARwas0.964±0.458and0.784±0.322respectively.Theyweresignifi—candyhigherthanthoseintheadjacenttissuesamplesand27normallivertissuesamples(P<0.05).Therelativeexpressionintensityofu—PAandu—PARwerealsosignificantlyhigherinthesubgr
7、oupwithcarcino—masoflargersizeandseveralinvasiveandmetastaticindexes(P<0.05).ConclusionsExpressionofuPAanduPARmRNAlevelswassignificantlyhigherinhepatocellularcarcinomatissuesamplesandinthesub—groupwithinvasiveandmetastaticindexesthaninadjacenttissuesamplesan
8、dnormallivertissuesamples,indicatingthattheymightplayimportantrolesinhepaticcarcinogenesis,invasivenessandmetastasis.【Keywords】Hepaticneoplasms;uPA;uPAR肿瘤细胞水解细胞外基质是肿瘤浸润和转移过程
