2、品含量测定;HPLC AbstractObjective:To establish a method for quantitative determination of adenosine in Ixeris by HPLC.Method:HPLC was performed on a column of Phenomenex Gemini C18 (250 mm ×4.6 mm,5 μm),and Acetonitrilewater (9∶91) as a mobilephase was used,de
3、tection wavelength at 260 nm and flow rate was 1.0 ml/min.Results:The method had good linear relationship with integral value and the peak area in the rage of 0.070~0.70 μ6g,the correlation coefficient (r2) was 0.9998. The average recovery rate was 97.6%,RS
4、D was 0.72% (n=5).Conclusion:The method is simple,accurate and reappearance. It can be used for quality control of Ixeris. KeywordsIxeris;adenosine;determination ofthe contentofsample;HPLC 苦碟子为菊科植物抱茎苦荬菜[Ixeris sonchifolia(Bge.)Hance] 的当年生干燥全草,分布我国的东北、内蒙古等