epo硬膜外途径干预大鼠脊髓损伤后caspase3及fasl表达与细胞凋亡的相关性研究

《epo硬膜外途径干预大鼠脊髓损伤后caspase3及fasl表达与细胞凋亡的相关性研究》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、EPO硬膜外途径干预大鼠脊髓损伤后caspase3及FasL表达与细胞凋亡的相关性研究.L.编辑。作者：陈淑强徐又佳俞晨郝彦明张振东顾永平【摘要】［目的］在大鼠急性脊髓损伤后不同时间硬膜外腔注射促红细胞生成素,观察并探讨其对脊髓神经功能的保护作用以及对脊髓神经细胞凋亡的影响。［方法］将48只体重（270±10)g成年雄性SpragueDaotorfunctionandapoptosisofneuralcell,afteriteinratsaticspinalcordinjury(SCI).［Method］Atotalof48adultmaleS

2、pragueDalydividedintofourgroups.Ratsinnormalgroup（n=6）andshamgroup(n=6)underinectomyproducureonly.Incontrolgroup,rats(n=18)recEIvednormalsalineepidurallyat1,6and24hoursafterinjury.Treatmentgroup(n=18)received5000iu/kgbodyaneerythropoietinadministeredepidurallyat1,6and24hoursa

3、fterinjury.SCIimpactaccordingtotheimprovedAllenmethod.Behavioralevaluationoftheratsade48hoursaftertraumausingtheBasso,Beattie,andBresnahan(BBB)scoringsystemandRivlin’stiltboardexperiment.Injuredspinalcordtissuecellapoptosisinedbytheterminaldeoxynucleotidaltransferasemediatedd

4、UTPbiotinnickendabeling(TUNEL)reactionat48hour.FaslandCaspase3expressioninedbyimmunohistochemicalanalysisat48hoursafterinjury.TheresultsicroscopeandanalyzedbySPSSstatisticssoftotorfunctionprovedat1,6,and24hoursafterinjuryintheexperimentgroup.Theindexesofneuralcelldecreasedsi

5、gnificantly(P<0.01).FaslandCaspase3expressedlessintheexperimentgroup.ThenumberofneuralcellsberofCaspase3(r=0.941)andFasl(r=0.929)expressedcells(P<0.01).［Conclusion］Analysisoftheresultsindicatedthatapoptosisplaysanimportantroleinthesecondaryspinalcordinjury.Activationof

6、FaslandCaspase3mayberelatedtocellsapoptosisintheinjuredspinalcord．EarlyapplicationofrhEPOcouldimprovetheneuromotorfunction.Theprotectionispartiallyduetothereductionofneuralcellapoptosis.Keyl/kg)腹腔注射麻醉。备皮后，俯卧位固定，消毒，铺盖无菌巾单。按照Nystrom法［8］，以T12为中心,切开皮肤和肌肉,长约2cm,显露T11～13棘突和椎板,咬除T12

7、棘突和椎板,以T12脊髓为中心显露大小为4mm×6mm的打击区。在硬膜表面垫一曲度与脊髓表面一致的塑料垫片，用直径2.5mm、重10g的圆柱状金属棒在细玻璃管的引导下从7cm高处垂直落下打击，造成以T12为中心的脊髓急性挫伤(致伤力为70g·cm)。损伤后每天3次人工按压膀胱排尿。假手术组仅行椎板切除。实验各组大鼠均肌注青霉素QD，分笼饲养。1.3硬膜外埋管术打击后行硬膜外埋管,将长约6cm、内径0.3mm、外径0.6mm的PE10聚乙烯管（宁波安来科技有限公司）直视下由伤口向大鼠尾端插入硬膜外腔约0.5cm，严密缝合伤口、肌肉层及筋膜层以防渗漏

8、并固定导管，然后从皮肤切口一侧用注射器针头引出，缝合伤口后用酒精灯烧灼末端封闭导管。1.4rhEPO干预方法分别于大鼠脊髓