人原发性胃恶性淋巴瘤裸小鼠原位移植模型的建立

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1、人原发性胃恶性淋巴瘤裸小鼠原位移植模型的建立作者：脱帅刘秋珍张宁朱希伟王明耀　【摘要】目的为探讨胃恶性淋巴瘤的发病机制和治疗方法提供理想的动物模型。方法采用人胃恶性淋巴瘤术中原发灶和肝转移灶新鲜瘤组织植入裸小鼠胃黏膜下层，观察原位移植成瘤率，移植瘤的侵袭和转移率，进行形态学、染色体核型和流式细胞分析。结果13例人胃恶性淋巴瘤标本9例移植成功。依据ethodsHistologicallyfreshprimaryandlivermetastaticlymphomatissuesremovedfrompatientsarygastricmalig

2、nantlymphomaucosaofnudemicetoobservetumorgenecity,invasionandmetastasisforanalysisofmorphologicalcharacteristics,karyotypeandfloetry.ResultsSuccessfultransplantationofhumanprimarygastricmalignantlymphomaalignantlymphoma,therescreenedthreestrainsofmodels,ie,orthotopictransp

3、lantationmodel(HGBL9903)ofhumanprimarygastricmalignantlymphoma(nonHodgkin'sBcell)innudemice,metastasismodel(HGBL9904)ofhumanprimarygastricmalignantlymphoma(nonHodgkin'sBcell)innudemiceandorthotopictransplantationmodel(HGBL9902)ofhumanprimarygastricmalignantlymphoma(Ho

4、dgkin'sBcell)innudemice.Immunohistochemistryshoberofchromosomefor7587.Floetrysho1.23to1.47,ie,heteroploid.Threemodelsiceorsthethirdgeneration.InHGBL9903,livermetastasisrate,spleenmetastasisrateandlymphnodemetastasisrateorsspontaneouslygreachofnudemicetoinfiltrateanddisru

5、pteverylayerofthegastricatogenousmetastasis(eg,liverandspleeninvolved),lymphnodemetastasisandceliacplantingmetastasisoccurred.ConclusionsThreesuccessfullyestablishedorthotopictransplantationmodels(HGBL9904,HGBL9903andHGBL9902)ofhumanprimarygastricmalignantlymphomainnude

6、miceareconsistentangastricmalignantlymphomainaspectsofhistopathologicalandultrastructuralfeatures,DNAcontentandchromosomalkaryotypeandhencepletelysimulatethenaturalclinicalprocessofhumanprimarygastricmalignantlymphoma.【Keyodel,animal;Gastriclymphoma;Malignantlymphoma;Tumor

7、transplantation;Nudemice为深入探讨胃恶性淋巴瘤的生物学行为，我们将人胃恶性淋巴瘤新鲜组织块移植于裸鼠胃黏膜下层，成功地建立了1株人原发性胃非霍奇金B细胞恶性淋巴瘤裸鼠原位移植肝转移模型HGBL9903；1株人原发性胃非霍奇金B细胞恶性淋巴瘤裸鼠原位移植模型HGBL9904；1株人原发性胃霍奇金B细胞恶性淋巴瘤裸鼠原位移植模型HGBL9902。现报道如下。1材料与方法1.1实验动物BALB/Cnu/nu裸小鼠由中国医学科学院肿瘤研究所动物室提供。鼠龄3～5周，体重17～20g，雌雄兼用，在无特殊病原体(spec

8、ificpathogenfree,SPF)的条件下饲养。1.2标本来源人胃恶性淋巴瘤新鲜标本取自我院和辽宁省肿瘤医院，共13例。其中非霍奇金B细胞弥漫性小裂细胞型4例，非霍奇金B