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ID:32021851
大小:2.08 MB
页数:31页
时间:2019-01-30
《【硕士论文】丙丁酚对急性痛风性关节炎的防治及作用机制研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、丙丁酚对急性痛风l生关节炎韵防治及作用5Ii兀蛊附f究摘要目的探讨丙丁酚防治急性痛风性关节炎的可行性及其可能的作用机制。方法1.60只Wistar雄性大鼠适应性喂养1周后,随机分为6组,对照组、模型组、丙丁酚高中低剂量组和秋水仙碱组。除对照组关节腔注射对照液外,其它各组大鼠单侧踝关节腔内注射尿酸钠晶体溶液建立急性痛风性关节炎模型,同时用药组给予药物灌胃3天,观察2、4、6、8、10、24、48和72小时时各组关节炎症、肿胀指数变化,同时比较72小时时各组大鼠关节液白细胞计数、白细胞介素-1B、丙二醛和超氧化物歧化酶的量。HE染色
2、观察大鼠关节滑膜组织炎症反应情况。2.用尿酸盐晶体刺激人单核细胞,用丙丁酚干预培养18小时后检测培养液中白细胞介素一1B的浓度。结果1.丙丁酚各剂量组关节炎症肿胀指数下降幅度由低剂量向高剂量依次增加,与模型组相比,中高剂量组在72小时下降程度较明显(P3、型组大鼠关节滑膜组织充血增生明显,且白细胞浸润增多明显,丙丁酚高剂量治疗组炎症明显减轻。2.丙丁酚各剂量组单核细胞分泌的白细胞介素一lB量较实验对照组无明显减少(P>O.05)。结论1.中高剂量的丙丁酚可以有效的控制大鼠急性痛风性关节炎的发作,其疗效具有一定的剂量依赖性。2.丙丁酚对炎症反应的第一步单核细胞摄取尿酸盐晶体后白细胞介素-1B的分泌无抑制作用,其可能主要是通过抑制炎症细胞向炎症部位的聚集及氧自由基的生成起作用。关键词:丙丁酚;痛风;关节炎;尿酸盐晶体;人单核细胞株;炎症介质RoleofProbucolinResolu4、tionofMonosodiumUrateMonohydrateCrystaIInducedAcuteGoutyArthritisofRatAbstractobjective:TostudythefeasibilityandmechanismofProbucolinresolutionofmonoso—diumuratemonohydrate(MSU)crystal—inducedarthritis.Methods:1.60Wistarmaleratswerefedadaptiveoneweek,andthenwererando5、mlydividedinto6groups:thecontrolgroup、modelgroup、High—Medium—LowdoseprobucolgroupsandColchicinegroup.AratmodelofacutegoutyarthritiswasinducedwiththeinjectionofMSUintoratanklejointcavity.Controlgroup,intra-articularinjectionofcontrolsolution,othersgroupswereinjectedMS6、U.Atthesametime,giventhedrugtreatmentgroupadministered3days.TheeffectsofProbucolonarthritiswere.investigatedthroughobservingclinicalfeaturesat2,4,6、10,24、48、72hours,andalsoobservingthesynovialfluidwhitebloodcellcount、interleukin—l13(IL-113)、MDAandSODat72hours.Itwasob7、servedthesynovialtissueinflammationbyHEstaining.2.IL一1BconcentrationofculturemediumWasobservedafterthel8hinterventionwithProbucolinTHP·1monocyteswhilestimulatedbyMSU.Results:1.ThedegreeofjointswellingdecreasedgraduallyfromProbucollow—dosegrouptohigh·-dosegroupandther8、ewassignifanctdifferencebetweenmid--doseand11i舯-doseProbucolgroupsandmodelgroupat72h(P<0.05).Thesynovialfluidwhitebloodcellcountand
3、型组大鼠关节滑膜组织充血增生明显,且白细胞浸润增多明显,丙丁酚高剂量治疗组炎症明显减轻。2.丙丁酚各剂量组单核细胞分泌的白细胞介素一lB量较实验对照组无明显减少(P>O.05)。结论1.中高剂量的丙丁酚可以有效的控制大鼠急性痛风性关节炎的发作,其疗效具有一定的剂量依赖性。2.丙丁酚对炎症反应的第一步单核细胞摄取尿酸盐晶体后白细胞介素-1B的分泌无抑制作用,其可能主要是通过抑制炎症细胞向炎症部位的聚集及氧自由基的生成起作用。关键词:丙丁酚;痛风;关节炎;尿酸盐晶体;人单核细胞株;炎症介质RoleofProbucolinResolu
4、tionofMonosodiumUrateMonohydrateCrystaIInducedAcuteGoutyArthritisofRatAbstractobjective:TostudythefeasibilityandmechanismofProbucolinresolutionofmonoso—diumuratemonohydrate(MSU)crystal—inducedarthritis.Methods:1.60Wistarmaleratswerefedadaptiveoneweek,andthenwererando
5、mlydividedinto6groups:thecontrolgroup、modelgroup、High—Medium—LowdoseprobucolgroupsandColchicinegroup.AratmodelofacutegoutyarthritiswasinducedwiththeinjectionofMSUintoratanklejointcavity.Controlgroup,intra-articularinjectionofcontrolsolution,othersgroupswereinjectedMS
6、U.Atthesametime,giventhedrugtreatmentgroupadministered3days.TheeffectsofProbucolonarthritiswere.investigatedthroughobservingclinicalfeaturesat2,4,6、10,24、48、72hours,andalsoobservingthesynovialfluidwhitebloodcellcount、interleukin—l13(IL-113)、MDAandSODat72hours.Itwasob
7、servedthesynovialtissueinflammationbyHEstaining.2.IL一1BconcentrationofculturemediumWasobservedafterthel8hinterventionwithProbucolinTHP·1monocyteswhilestimulatedbyMSU.Results:1.ThedegreeofjointswellingdecreasedgraduallyfromProbucollow—dosegrouptohigh·-dosegroupandther
8、ewassignifanctdifferencebetweenmid--doseand11i舯-doseProbucolgroupsandmodelgroupat72h(P<0.05).Thesynovialfluidwhitebloodcellcountand
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