风湿康冲剂治疗膝骨关节炎的实验分析

《风湿康冲剂治疗膝骨关节炎的实验分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、硕士学位论文晰。风湿康低剂量组与模型组基本相似。风湿康中剂量组软骨表面欠光滑，软骨细胞变性，基质变性。双醋瑞英组四层结构基本清晰，潮线基本完整，少许关节软骨细胞有轻度变性。风湿康高剂量组关节软骨内有少许炎细胞浸润，关节软骨细胞有轻度变性，潮线基本完整。3．4免疫组化结果正常对照组未见MMP．13的表达：模型组中全层软骨细胞质中可见MMP．13的表达，为深棕褐色颗粒；风湿康冲剂低剂量组也几乎全层表达；风湿康冲剂中剂量组在关节软骨表层和移行层表达，为浅棕褐色颗粒，风湿康冲剂高剂量_．组和双醋瑞英组只在关节软骨表层可见少许棕褐色颗粒。

2、与模型组比较，一风湿康冲剂中剂量和高剂量组均可显著抑制MMP．13在膝关节软骨中的表达(P<0．001)，风湿康冲剂高剂量组与双醋瑞英组在抑制MMP．13在膝关节软骨中的表达方面，P>O．05，差异无统计学意义。四、结论风湿康可能是通过降低血清中IL．1p的水平，升高血清中TGF邛1的水平，减少MMP．13在关节软骨中的表达，从而对骨关节炎发挥治疗作用。其中风湿康冲剂高剂量组较低中剂量组在抗炎和软骨保护方面有显著的优势。关键词风湿康骨关节炎IL-113TGF一13l删P-13V硕士学位论文Effectoffengshikango

3、nexperimentalosteoarthritisinratsName：QiJing．ming一Supervisor：Pro．WUQi—fuABSTRACT1．0bjectivesandsignificanceOsteoarthritiswhichwasalsocalledRegressiveGonarthrosis，asaheterogeneousgroupofdiseaseswhosethesymptomsandsignsresultedfromthedestroyedintegrityofarticularcartil

4、agesandthelesionofsubchondralbones．Accordingtothedistributionofjoint，OsteoarthritisclassifiedsystemicOsteoarthritisandlimitedOsteoarthritis．KneeOsteoarthritis(KOA)asarepresentativeoflimitedOsteoarthritiswhichWascharacterizedbyjointpain，jointrigid，campaignwaslimited，n

5、oisyoffriction．Aftermiddleage，TheincidenceofthediseaseWashigh．TheoccurrenceofOAWasincreased诵thage，whichcausedseriousdamagetothehealthofthemiddleagedandoldpeople．TheetiologyofKOAremainedunclear,whichWasgenerallyacknowledgedthatbiomechanicsfactors，descendiblityfactorsa

6、ndexogenousfactorscausedthechangesinmetabolismofsynovium，cartilageandbone，thusproducedinflammation,structuredestruction．ThefundamentalpathologicalchangeWasarticularcartilagedegeneration．ModemmedicinebelievedthatthecartilagedegenerationWasanimportantaspect．Proveninman

7、ystudies，cartilagedegenerationWasrelatedtoaberrantexpressionofCytokine．Innormalcircumstances，thedecomposableCytokineandthegrowfactormaintainedthebalancebetweenthecatabolismandthemetabolismofarticularcartilagematrix．TheABSTRACTdecomposableCytokine，suchasIL-I，whichchie

8、flystimulatedthespecialmatrixmetalloprotcinases(MMP)production，causedmatrixdegradation，stimulatedsynoviocytesproliferationanddegene