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1、Huangetal.BMCInfectiousDiseases2013,13:437http://www.biomedcentral.com/1471-2334/13/437TECHNICALADVANCEOpenAccessAneasyoperatingpathogenmicroarray(EOPM)platformforrapidscreeningofvertebratepathogens1†2†34445WeiweiHuang,YinhuiYang,XinleiZhang,ChanganZhao,AihuaYin,XiaozhuangZhang,ZhengxinHe,2*1*Y
2、ongqiangJiangandLiangZhangAbstractBackground:InfectiousdiseasesemergefrequentlyinChina,partlybecauseofitslargeandhighlymobilepopulation.Therefore,arapidandcost-effectivepathogenscreeningmethodwithbroadcoverageisrequiredforpreventionandcontrolofinfectiousdiseases.Theavailabilityofalargenumberofm
3、icrobialgenomesequencesgeneratedbyconventionalSangersequencingandnextgenerationsequencinghasenabledthedevelopmentofahigh-throughputhigh-densitymicroarrayplatformforrapidlarge-scalescreeningofvertebratepathogens.Methods:Aneasyoperatingpathogenmicroarray(EOPM)wasdesignedtodetectalmostallknownpath
4、ogensandrelatedspeciesbasedontheirgenomicsequences.ForeffectiveidentificationofpathogensfromEOPMdata,astatisticalenrichmentalgorithmhasbeenproposed,andfurtherimplementedinauser-friendlyweb-basedinterface.Results:Usingmultipleprobesdesignedtospecificallydetectamicrobialgenusorspecies,EOPMcancorr
5、ectlyidentifyknownpathogensatthespeciesorgenuslevelinblindedtesting.DespitealowersensitivitythanPCR,EOPMissufficientlysensitivetodetectthepredominantpathogenscausingclinicalsymptoms.DuringapplicationintworecentclinicalinfectiousdiseaseoutbreaksinChina,EOPMsuccessfullyidentifiedtheresponsiblepat
6、hogens.Conclusions:EOPMisaneffectivesurveillanceplatformforinfectiousdiseases,andcanplayanimportantroleininfectiousdiseasecontrol.Backgroundbyaworldwideepidemic.Duringtheepidemic,66%oftheThefrequentinvasionofmicroorganisms,includingvi-SARScaseswerereportedinChina,resultingin349humanruses,bacter
7、ia,fungi,parasites,andothereukaryoticanddeaths[2].In2007,anoutbreakofhand,foot,andmouthprokaryoticorganisms,hasthreatenedandwillcontinuedisease(HFMD)infected1149personsandcausedthetothreatenthelifeandhealthofhumansandotherdeathoft
